Anti-NOLC1 Rabbit Polyclonal Antibody for WB - Q14978

Core Product Specifications and Parameters

Biological Background: NOLC1 Function and Localization

• Acting as a regulator of RNA polymerase I, NOLC1 connects RNA polymerase I with enzymes responsible for ribosomal processing and modification, playing a critical role in ribosome biogenesis. Related references: PMID:10567578 PMID:26399832
• Following monoubiquitination by the BCR(KBTBD8) complex, NOLC1 associates with TCOF1 to remodel the translational program, a process required for neural crest specification. Related references: PMID:26399832
• It is involved in nucleologenesis, likely contributing to the structural integrity of the nucleolar fibrillar center and dense fibrillar component. Related references: PMID:9016786
• NOLC1 possesses intrinsic GTPase and ATPase activities, suggesting a role in nucleotide-dependent processes within the nucleolus. Related references: PMID:9016786
• The protein localizes predominantly to the nucleolus, but is also detected in the nucleus and cytoplasm.
• Subject to multiple post-translational modifications, including acetylation, methylation, phosphorylation, and isopeptide bond formation (ubiquitination), which regulate its function.
• Alternative splicing of NOLC1 mRNA generates several isoforms, potentially expanding its functional repertoire.
• Also referred to as hepatitis C virus NS5A-transactivated protein 13 (NS5ATP13), linking it to viral host-factor interactions.

Experimental Guidance and Technical Tips

• The immunogen encompasses the central region (aa 400-699) of human NOLC1, which is less conserved than the N-terminal repeat domain; cross-reactivity with mouse and rat suggests epitope conservation across species.
• For Western blot (WB), a band near 74 kDa (predicted molecular weight) is expected; additional bands may arise due to post-translational modifications or isoforms.
• For immunohistochemistry (IHC-P), antigen retrieval methods may be required to expose epitopes in formalin-fixed tissues; nucleolar staining should be observed.
• In immunofluorescence (IF/ICC), NOLC1 typically shows nucleolar localization with possible nucleoplasmic and cytoplasmic signals.
• ELISA can be used for quantitative detection; consider pairing with a capture antibody for sandwich ELISA applications.
• Since NOLC1 undergoes various modifications, consider the biological context when interpreting results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. For studies on nucleolar biology and RNA polymerase I regulation, we offer validated primary antibodies, antibody pairs, and ancillary reagents to support your assay development and research needs. Contact our team for bulk orders or custom sourcing requests.