Anti-NOLC1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14978

Core Product Specifications and Parameters

Biological Background: NOLC1 Function and Localization

• NOLC1 (Nucleolar and coiled-body phosphoprotein 1), also known as Nopp140, p130, NOPP130, NOPP140, or NS5ATP13, is encoded by the NOLC1 gene and undergoes alternative splicing.
• Functions as a regulator of RNA polymerase I by linking RNA polymerase I with enzymes responsible for ribosomal RNA processing and modification. Related references: PMID:10567578, PMID:26399832
• Plays a critical role in neural crest specification: upon monoubiquitination by the BCR(KBTBD8) complex, NOLC1 associates with TCOF1, serving as a platform to connect RNA polymerase I with the ribosomal processing machinery, thereby remodeling the translational program to favor neural crest differentiation. Related references: PMID:26399832
• Involved in nucleologenesis by maintaining the structural integrity of the fibrillar center and dense fibrillar component within the nucleolus; exhibits intrinsic GTPase and ATPase activities. Related references: PMID:9016786
• Localizes predominantly to the nucleolus, but is also found in the nucleus and cytoplasm.
• Subject to multiple post-translational modifications, including acetylation, methylation, phosphorylation, and ubiquitination; contains ATP- and GTP-binding domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human NOLC1; consider this when selecting detection strategies.
• Suitable for Western blot (WB), immunohistochemistry-paraffin (IHC-P), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. Validate in your specific sample system and optimize antibody dilutions.
• Cross-reactivity is confirmed for human and mouse; potential reactivity with other species should be verified experimentally.
• For nucleolar targets, ensure proper nuclear permeabilization and include appropriate controls to confirm localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and institutions a convenient one-stop procurement platform for IVD raw materials, technical services, and consulting needs—from early concept to clinical application. This anti-NOLC1 monoclonal antibody supports investigations into nucleolar biology, ribosomal RNA processing, and neural crest development. Beyond this product, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.