Anti-NOL6 Rabbit Polyclonal Antibody for WB, ELISA - Q9H6R4

Core Product Specifications and Parameters

Biological Background: NOL6 Function and Localization

• NOL6, also known as Nucleolar RNA-associated protein (NRAP) and UTP22, is a vital component of the small subunit (SSU) processome, the earliest precursor of the small eukaryotic ribosomal subunit.
• It participates in the nucleolar assembly of the SSU processome where multiple ribosome biogenesis factors, chaperones, and ribosomal proteins coordinate pre-rRNA folding, modification, cleavage, and targeted degradation by the RNA exosome.
• The protein localizes to the nucleus, nucleolus, and chromosome, consistent with its role in ribosome biogenesis and chromatin-associated functions.
• NOL6 is classified as an RNA-binding protein and contains a coiled-coil domain, suggesting a structural or scaffolding role in ribonucleoprotein complexes.
• Post-translational modifications include phosphorylation (indicated by keyword Phosphoprotein), which may regulate its activity during the cell cycle or stress.
• Alternative splicing of the NOL6 gene produces multiple isoforms, potentially contributing to functional diversity.
• As a member of the reference proteome, NOL6 is broadly conserved and extensively studied in eukaryotes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 1032-1146); consider using this sequence to confirm immunoreactivity in your system if needed.
• For Western blot, expect a band around 128 kDa. Validate specificity with appropriate positive and negative controls.
• Using nuclear or nucleolar-enriched lysates can enhance signal detection for this nucleolar protein.
• For ELISA, the antibody may be suitable for capture or detection in a sandwich format, but thorough optimization is required.
• As a polyclonal antibody, be aware of potential batch-to-batch variability; consider lot-specific validation.
• Cross-reactivity with mouse and rat NOL6 allows application in rodent models; verify expected band size in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers and research laboratories worldwide. Our anti-NOL6 polyclonal antibody, targeting a key nucleolar ribosome biogenesis factor, fits into a broad portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material procurement. We streamline your development pipeline from concept to clinic, ensuring reliable supply and technical support for nucleolar biomarker research and assay development.