Anti-NOL3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O60936

Core Product Specifications and Parameters

Biological Background: Nucleolar protein 3 (NOL3) Function and Localization

• NOL3 (Nucleolar protein 3), also known as apoptosis repressor with CARD (ARC) or Nop30, is a multifunctional protein encoded by the NOL3 gene in human. It belongs to the apoptosis repressor family and contains a CARD domain.
• NOL3 blocks the extrinsic apoptotic pathway by interfering with death-inducing signaling complex (DISC) assembly through interactions with FAS and FADD, and by limiting CASP8 availability via phosphorylation-dependent mitochondrial localization (By similarity).
• In the intrinsic pathway, NOL3 directly binds to BAX, preventing its activation, mitochondrial dysfunction, and the release of pro-apoptotic factors. Related references: PMID:15004034
• NOL3 acts as a cytosolic calcium buffer, dissociating from CASP8 to maintain calcium homeostasis, thereby inhibiting calcium-mediated cell death. Related references: PMID:15509781
• Additional anti-apoptotic roles include suppression of oxidative stress-induced apoptosis (via blocking CASP2 activation and BAX translocation), hypoxia-induced apoptosis (inhibition of cytochrome c release), and TNF-induced necrosis (by abrogating RIPK1 recruitment to complex I) (By similarity).
• Subcellular localization includes nucleolus, cytoplasm, mitochondria, sarcoplasmic reticulum, and membrane, consistent with its diverse functions in RNA splicing and cell death.
• Tissue specificity: highly expressed in heart and skeletal muscle; detected at low levels in placenta, liver, kidney, and pancreas.

Experimental Guidance and Technical Tips

• For Western blot (WB), a specific band at approximately 23 kDa is expected under reduced denaturing conditions. Heart or skeletal muscle lysates are recommended as positive controls.
• In immunohistochemistry (IHC-P), antigen retrieval may be required. Positive staining should be observed in cardiomyocytes or skeletal muscle fibers; consider using isotype controls to confirm specificity.
• For immunofluorescence (IF/ICC), note that NOL3 exhibits both nuclear and cytoplasmic staining. Co-staining with organelle markers (e.g., mitotracker for mitochondria) can help confirm subcellular targeting.
• When setting up ELISA, the full-length recombinant immunogen can serve as a standard; antibody dilution should be optimized empirically for the specific assay format.
• Due to the polyclonal nature, lot-to-lot consistency should be monitored; initial validation in the relevant sample system is advised before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a comprehensive range of IVD raw materials, including validated antibodies for apoptosis and cell death research. In addition to this anti-NOL3 polyclonal antibody, we supply optimized monoclonal/polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to accelerate your assay development. Our global sourcing capabilities extend to rare and emerging targets, providing a reliable one-stop resource from concept to clinical application.