Anti-NOL3 Polyclonal Antibody for WB, IF/ICC, ELISA - O60936

Core Product Specifications and Parameters

Biological Background: NOL3 Function and Localization

• NOL3, also known as ARC, FCM, MYP, or NOP30, is a nucleolar protein that functions as an apoptosis repressor, blocking multiple cell death pathways.
• It inhibits extrinsic apoptosis by interacting with FAS and FADD to block DISC assembly, and by sequestering CASP8 in a phosphorylation-dependent manner at mitochondria.
• In the intrinsic pathway, NOL3 binds to BAX, preventing its activation, mitochondrial dysfunction, and release of pro-apoptotic factors. Related references: PMID:15004034
• NOL3 acts as a cytosolic calcium buffer, disrupting the CASP8 interaction and preserving calcium homeostasis to inhibit calcium-mediated cell death. Related references: PMID:15509781
• It negatively regulates apoptosis induced by oxidative stress and hypoxia by blocking CASP2 activation, BAX translocation, and cytochrome c release.
• NOL3 also suppresses TNF-induced necrosis by interacting with TNFRSF1A and preventing RIPK1 recruitment to complex I.
• Subcellularly, NOL3 localizes to the nucleus, nucleolus, cytoplasm, mitochondria, sarcoplasmic reticulum, and membrane, reflecting its multifunctional roles.
• Highly expressed in heart and skeletal muscle, with lower levels in placenta, liver, kidney, and pancreas, suggesting tissue-specific apoptosis regulation.

Experimental Guidance and Technical Tips

• For WB, the expected band is at ~23 kDa; consider using appropriate positive and negative controls to validate specificity.
• In IF/ICC, NOL3 exhibits a complex localization pattern; ensure fixation and permeabilization conditions are optimized for your cell type and compartment of interest.
• For ELISA, a recombinant NOL3 protein standard is recommended for quantitative assessments; cross-reactivity with mouse NOL3 should be verified in your assay system.
• As a polyclonal antibody, it may recognize multiple epitopes; lot-to-lot consistency should be monitored in critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with integrated access to high-quality IVD raw materials, including validated antibodies like this anti-NOL3 polyclonal antibody for apoptosis research. Whether you need bulk quantities of polyclonal antibodies, matched antibody pairs, or ancillary reagents, our streamlined sourcing platform supports your workflow from early-stage development to clinical application.