Anti-NOL12 Rabbit Polyclonal Antibody for WB, ELISA - Q9UGY1

Core Product Specifications and Parameters

Biological Background: NOL12 Function and Localization

• NOL12 (Nucleolar protein 12) is a multifunctional RNA-binding protein encoded by the NOL12 gene, featuring a coiled-coil domain and capable of binding both RNA and rRNA (UniProt: Q9UGY1).
• Plays a critical role in the resolution of DNA stress and maintenance of genome integrity by localizing to sites of DNA damage.
  Related references:
  PMID:29069457
• Regulates nucleolar organization, limiting nucleolar size and controlling nucleolar number, which is essential for proper ribosome biogenesis.
  Related references:
  PMID:30988155
• Modulates the nucleolar levels of fibrillarin and nucleolin, two key proteins involved in pre-rRNA processing and ribosome assembly, thereby influencing ribosome production.
• Subcellular localization is primarily in the nucleolus and nucleus, but also detected in the cytoplasm, indicating dynamic shuttling (UniProt).
• The protein has a calculated molecular weight of approximately 24.7 kDa (observed ~25 kDa in experiments), consistent with the 213-amino acid full-length protein.
• Classified under reference proteome and identified in proteomics studies, with functional links to chromatin maintenance and RNA metabolism.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human NOL12 protein (aa 1-213), which may contribute to high specificity in Western blot; an expected band near 25 kDa should be observed in lysates from human cells or tissues.
• For ELISA applications, consider optimizing coating concentrations and using appropriate blocking buffers to minimize background, as polyclonal antibodies can exhibit batch-dependent variability.
• Validate the antibody in your specific sample system, as cross-reactivity with other species or isoforms has not been extensively tested.
• When performing Western blot, include appropriate loading controls and molecular weight markers to confirm band identity.
• Due to NOL12's nucleolar localization, subcellular fractionation may enrich the signal and aid in detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, from validated antibody pairs to optimized monoclonal and polyclonal antibodies like this anti-NOL12 reagent. We support projects in nucleolar biology and RNA metabolism by offering bulk ancillary reagents and sourcing solutions for rare targets, ensuring a reliable supply chain for your assay development needs. Partner with us to streamline your journey from concept to clinic.