Anti-NOL10 (PQBP5) Polyclonal Antibody for WB, ELISA - Q9BSC4

Core Product Specifications and Parameters

Biological Background: NOL10 Function and Localization

• NOL10 (Nucleolar protein 10), also known as PQBP5, is a protein encoded by the NOL10 gene in humans. It is a reference proteome member and characterized by the presence of WD repeats and a coiled coil domain.
• Subcellularly, NOL10 localizes specifically to the nucleolus within the nucleus, consistent with its role in nucleolar processes.
• The protein is subject to post-translational modifications, including phosphorylation and acetylation, which may regulate its function and interactions.
• Alternative splicing produces multiple isoforms of NOL10, potentially expanding its functional versatility.
• With a molecular weight of approximately 80 kDa (calculated ~80,302 Da), NOL10 has been identified through proteomics identification studies.
• The 3D structure of NOL10 has been determined, providing insights into its structural organization and potential binding surfaces.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 300-400) of human NOL10, suggesting the antibody recognizes a conserved internal epitope. Users may consider epitope exposure conditions in their assays.
• This antibody is suitable for Western blotting (WB) and ELISA. For WB, a band around 80 kDa is expected; validate in the relevant sample system (e.g., human or mouse cell lysates) under optimized conditions.
• Due to reported human and mouse cross-reactivity, the antibody may be used for studies involving these species. Confirm reactivity in your specific experimental model.
• As a polyclonal antibody, it offers robust signal amplification but may require careful titration and appropriate negative controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a wide range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. Supporting nucleolar biology and protein interaction studies, this NOL10 antibody fits into our comprehensive catalog designed to accelerate research and assay development. For custom sourcing or technical consultation, reach out to leverage our integrated supply chain and expertise.