Anti-Nogo (RTN4) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9NQC3

Core Product Specifications and Parameters

Biological Background: Nogo Function and Localization

• Reticulon-4 (RTN4), also known as Nogo, is an endoplasmic reticulum (ER) membrane protein that induces the formation and stabilization of ER tubules, regulating membrane morphogenesis.
  Related references: PMID:24262037, PMID:25612671, PMID:27619977
• It also influences nuclear envelope expansion, nuclear pore complex formation, and proper localization of inner nuclear membrane proteins.
  Related references: PMID:26906412
• Nogo acts as a developmental neurite growth regulatory factor, negatively regulating axon-axon adhesion and growth while facilitating neurite branching.
  Related references: PMID:10667797, PMID:11201742
• It inhibits central nervous system angiogenesis by restricting the spreading, migration, and sprouting of brain microvascular endothelial cells.
• Nogo modulates vascular remodeling, endothelial sphingolipid biosynthesis, and blood pressure regulation.
• In the immune system, it facilitates leukocyte transendothelial migration, TLR9 trafficking to endolysosomes, and cytokine expression during inflammation and tissue repair.
• Isoform B and isoform C inhibit BACE1 activity and amyloid precursor protein processing.
  Related references: PMID:16965550
• Nogo sequesters the anti-apoptotic proteins Bcl-xl and Bcl-2 to the ER, reducing their protective activity.
  Related references: PMID:11126360
• Multiple isoforms exhibit tissue-specific expression: isoform A in brain and testis; isoform B widely expressed except liver; isoform C in brain, skeletal muscle, and adipocytes; isoform D testis-specific.
• Subcellularly, Nogo localizes to the endoplasmic reticulum membrane, cell membrane, cell junctions, and synapses.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 60–160 of human Nogo (NP_065393.1), a region that may be conserved across some isoforms. Consider validating isoform-specific detection in your cell or tissue model.
• For Western blot (WB), a predominant band around 130 kDa is expected, but isoform sizes may vary. Use appropriate positive controls (e.g., brain lysate) and optimize antibody dilution.
• For immunofluorescence/ICC, permeabilization is recommended to access the ER membrane localization. Co-staining with an ER marker can confirm target specificity.
• The antibody shows cross-reactivity with human, mouse, and rat samples; always perform a species-specific validation when switching experimental systems.
• In ELISA, pair this antibody with a suitable capture/detection system; preliminary checkerboard titrations are advised to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a seamless supply chain for IVD raw materials, from discovery to commercialization. For neuroscience and vascular biology targets like Nogo, we can source validated antibody pairs, recombinant antigens, and ancillary reagents tailored to your assay development needs. Whether you require rare-target monoclonal antibodies or bulk ancillary materials, our technical and logistical support helps you accelerate your project with reliable, quality-assured components.