Anti-NOGO Receptor 1 (RTN4R) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BZR6

Core Product Specifications and Parameters

Biological Background: NOGO Receptor 1 Function and Localization

• Reticulon-4 receptor (RTN4R, also known as Nogo receptor) is a GPI-anchored glycoprotein that serves as a receptor for myelin-associated inhibitors, including Nogo (RTN4), OMG, and MAG, thereby mediating axonal growth inhibition and limiting regeneration in the adult CNS. Related references: PMID:12037567 PMID:12068310 PMID:12089450 PMID:12839991 PMID:19052207 PMID:28892071
• RTN4R also binds sialylated gangliosides GT1b and GM1, and by similarity, chondroitin sulfate proteoglycans and heparin, broadening its ligand repertoire. Related references: PMID:18411262
• Intracellular signaling is mediated through the coreceptor NGFR, leading to Rho activation, actin cytoskeleton reorganization, and growth cone collapse, which underlies its role in restricting dendritic spine and synapse formation. Related references: PMID:12426574 PMID:16712417 PMID:22325200
• RTN4R is essential for postnatal brain development, including axon migration across the midline and corpus callosum formation, and provides apoptotic protection to motoneurons, likely via MAG interaction.
• Subcellularly, RTN4R localizes to the cell membrane, membrane rafts, and is enriched in dendrites, axons, and the perikaryon, aligning with its role in axonal guidance.
• Tissue expression is widespread in the brain, with highest levels in gray matter, while low in heart and kidney; it is absent from oligodendrocytes.
• Post-translational features include a leucine-rich repeat domain, disulfide bonds, and a C-terminal GPI anchor, classifying it as a lipid-binding lipoprotein receptor. Additionally, it acts as a receptor for reovirus type 3 during microbial infection.

Experimental Guidance and Technical Tips

• Given that the immunogen corresponds to the C-terminal region (aa 244–473) of human RTN4R, this antibody may detect full-length and possible cleavage products. For Western blot, the predicted molecular weight is ~51 kDa; we recommend using brain tissue or neuronal cell lysates as positive controls.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to preserve membrane-associated RTN4R localization. Co-staining with dendritic or axonal markers may aid interpretation.
• The antibody has demonstrated reactivity with human, mouse, and rat samples; however, we advise validating the antibody in your specific model system, preferably using knockdown or knockout controls, to ensure specificity.
• ELISA applications can be developed using recombinant antigen as a standard; titration of both capture and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-RTN4R polyclonal antibody as part of a comprehensive portfolio of IVD raw materials for neuroscience and axon regeneration research. In addition to primary antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to support your full development pipeline. Our one-stop sourcing model ensures you have seamless access to high-quality reagents, accelerating your journey from concept to clinic.