Anti-NOGO Receptor 1 (RTN4R) Monoclonal Antibody for WB, ELISA - Q9BZR6

Core Product Specifications and Parameters

Biological Background: NOGO Receptor 1 (RTN4R) Function and Localization

• Reticulon-4 receptor (RTN4R), also known as NOGO Receptor 1, Nogo-66 receptor, or NgR, is encoded by the RTN4R gene in humans.
• Functions as a receptor for myelin-associated inhibitors RTN4, OMG, and MAG, as well as sialylated gangliosides GT1b and GM1, with intracellular signaling via the coreceptor NGFR. Related references: PMID:12037567 PMID:12068310 PMID:12089450 PMID:18411262 PMID:12426574
• Mediates axonal growth inhibition and plays a critical role in regulating axon regeneration and neuronal plasticity in the adult central nervous system. Related references: PMID:12839991 PMID:19052207 PMID:28892071
• Intracellular signaling triggers Rho activation and actin cytoskeleton reorganization; contributes to the restriction of dendritic spines and synapses during brain development. Related references: PMID:16712417 PMID:22325200
• Subcellularly, it is found at the cell membrane and in membrane rafts, with enrichment on dendrites, axons, and the perikaryon.
• Expression is widespread in the brain, with highest levels in gray matter; low levels are detected in heart and kidney, and it is absent from oligodendrocytes.
• Contains leucine-rich repeats, a GPI-anchor, and disulfide bonds, with a molecular weight of approximately 51 kDa (predicted 50708 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within amino acids 300-400 of human RTN4R; the antibody likely targets an internal epitope, so consider this in detection strategy design.
• For Western blot, expect a band near 51 kDa; optimize gel percentage and validate with a relevant sample system (e.g., brain tissue lysate) to confirm specificity.
• In ELISA, carefully optimize coating and detection conditions; cross-reactivity with mouse enables translational research applications.
• As a monoclonal antibody, it offers consistent lot-to-lot performance; perform initial titration for each assay system to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with a comprehensive portfolio of IVD raw materials and technical services. For neurobiology and axon regeneration research focused on NOGO Receptor 1, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Explore our one-stop procurement solutions to streamline your assay development from concept to clinical application.