Anti-NODAL Polyclonal Antibody for WB, ELISA - Q96S42

Core Product Specifications and Parameters

Biological Background: NODAL Function and Localization

• NODAL encodes the Nodal homolog, a secreted cytokine-like growth factor essential for embryonic development.
• It is critical for mesoderm formation and axial patterning during early embryogenesis.
• The protein is synthesized as a precursor and undergoes cleavage at a pair of basic residues to produce the mature signaling molecule.
• NODAL is a glycoprotein with conserved disulfide bonds, characteristic of secreted growth factors.
• Subcellularly, NODAL is secreted into the extracellular space to mediate paracrine signaling events.
• Mutations in NODAL are associated with heterotaxy, a congenital disorder affecting left-right body axis determination.
• Three-dimensional structural studies have revealed a cystine-knot fold in the mature domain, as documented in the UniProt record.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 198–347 of human NODAL, which includes part of the prodomain and mature domain; consider this when interpreting results in assays that distinguish precursor and cleaved forms.
• For Western blot, a band near 40 kDa may be observed under reducing conditions, but note that the secreted mature Nodal is smaller due to proteolytic processing.
• In ELISA applications, the antibody may be used for detection of recombinant or endogenous NODAL; validate in your specific sample matrix and format.
• Cross-reactivity with rat indicates a conserved epitope; testing in the intended rodent model is recommended to confirm reactivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD raw material procurement, supporting your transition from concept to clinical application. We offer reliable access to validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-NODAL product, and bulk ancillary reagents for immunoassay development. Our team also excels in sourcing rare and hard-to-find targets, providing a single convenient channel for all your IVD material needs in developmental biology research.