Anti-NOD2 Rabbit Polyclonal Antibody for WB - Q9HC29

Core Product Specifications and Parameters

Biological Background: NOD2 Function and Localization

• Nucleotide-binding oligomerization domain-containing protein 2 (NOD2, also known as CARD15) is a pattern recognition receptor that detects bacterial peptidoglycan fragments, specifically muramyl dipeptide (MDP) and its phosphorylated form 6-O-phospho-MDP, playing a central role in gastrointestinal immunity. Related references: PMID:12514169 PMID:12527755 PMID:12626759 PMID:36002575
• Upon MDP binding, NOD2 oligomerizes and recruits the adapter protein RIPK2, leading to NF-kappa-B and MAP kinase pathway activation and transcription of immune response genes. Related references: PMID:11087742 PMID:17355968 PMID:21887730 PMID:15198989
• NOD2 also functions in antiviral immunity by sensing single-stranded RNA from viruses, leading to IRF3 activation and type I interferon expression via interaction with MAVS. Related references: PMID:19701189
• In dendritic cells, NOD2 regulates autophagy by recruiting ATG16L1 to sites of bacterial entry, a mechanism that also supports intestinal stem cell survival through mitophagy. Related references: PMID:20637199
• NOD2 is involved in the endoplasmic reticulum stress response by sensing sphingosine-1-phosphate, which triggers inflammation via NF-kappa-B and MAP kinase signaling. Related references: PMID:27007849 PMID:33942347
• This protein can activate CASP1 and IL1B release in macrophages through NLRP1 activation following MDP stimulation. Related references: PMID:18511561
• NOD2 localizes to the basolateral cell membrane, cytoplasm, and mitochondria, and is highly expressed in monocytes, macrophages, dendritic cells, hepatocytes, and intestinal Paneth cells.
• Mutations in NOD2 are associated with inflammatory bowel diseases such as Crohn's disease and Blau syndrome, highlighting its critical role in immune homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–216, which encompass the N-terminal CARD domains; therefore the antibody likely recognizes both full-length NOD2 and any N-terminal fragments.
• For Western blot, the predicted molecular weight is ~115 kDa, but alternative initiation isoforms or post-translational modifications may produce additional bands. Validate in the relevant sample system and consider using appropriate controls.
• In immunohistochemistry and immunocytochemistry, optimize permeabilization and fixation conditions as NOD2 can be present at the membrane or in the cytoplasm.
• For ELISA applications, confirm the antibody's performance with the appropriate recombinant protein or peptide standard.
• When using this antibody in mouse or rat tissues, cross-reactivity is expected, but independent validation is recommended due to species-specific epitope variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing reliable access to IVD raw materials, technical services, and consultancy. This anti-NOD2 polyclonal antibody is part of our extensive portfolio of validated antibodies for innate immunity and inflammatory disease research. As your strategic partner, we also offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and customized rare-target sourcing to accelerate your assay development from concept to clinic.