Anti-NOD2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9HC29

Core Product Specifications and Parameters

Biological Background: NOD2 Function and Localization

• NOD2 is a pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments, primarily muramyl dipeptide (MDP), triggering innate immune responses (PubMed:12514169, PubMed:12527755, PubMed:12626759). Related references: PMID:12514169, PMID:12527755, PMID:12626759
• Upon MDP binding, NOD2 oligomerizes and recruits RIPK2, leading to NF-kB and MAPK activation and transcriptional regulation of immune genes (PubMed:11087742, PubMed:17355968, PubMed:21887730). Related references: PMID:11087742, PMID:17355968, PMID:21887730
• NOD2 plays a critical role in gastrointestinal immunity, maintaining intestinal microbiota balance and preventing inflammation; mutations are linked to inflammatory bowel disease (IBD) (PubMed:15198989). Related references: PMID:15198989
• Located at the cell membrane, basolateral membrane, cytoplasm, and mitochondrion, NOD2's localization is vital for its diverse signaling functions.
• Expressed in monocytes, macrophages, dendritic cells, Paneth cells, and intestinal stem cells, with higher levels in leukocytes.
• Also involved in antiviral responses by sensing viral ssRNA and interacting with MAVS to induce type I interferon (PubMed:19701189). Related references: PMID:19701189
• Regulates autophagy by interacting with ATG16L1, facilitating bacterial clearance and mitophagy in intestinal stem cells (PubMed:20637199). Related references: PMID:20637199
• Senses endoplasmic reticulum stress via sphingosine-1-phosphate, further activating NF-kB and MAPK pathways (PubMed:27007849, PubMed:33942347). Related references: PMID:27007849, PMID:33942347

Experimental Guidance and Technical Tips

• The immunogen (aa 28-270) covers the N-terminal tandem CARD domains of human NOD2, which mediate downstream signaling; consider this when interpreting results in functional assays.
• For Western blotting, the predicted molecular weight is ~115 kDa; post-translational modifications such as glycosylation and ubiquitination may cause band shifts.
• For immunofluorescence/ICC, permeabilization is required to detect cytoplasmic and membrane-associated NOD2; validate localization in the relevant cell type (e.g., macrophages, intestinal epithelial cells).
• For ELISA, this polyclonal antibody can be used as a capture or detection reagent; titrate to determine optimal working concentrations.
• Cross-reactivity with mouse NOD2 is reported; validate in mouse samples if using for cross-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibodies and reagents spanning innate immunity and inflammatory disease targets like NOD2. In addition to this high-quality anti-NOD2 polyclonal antibody, CamelBio can supply optimized antibody pairs, bulk ancillary reagents, and customized rare-target sourcing to accelerate your diagnostic development from concept to clinic.