Anti-NOD1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y239

Core Product Specifications and Parameters

Biological Background: NOD1 Function and Localization

• NOD1 (CARD4) is a pattern recognition receptor (PRR) belonging to the NOD-like receptor family, playing a crucial role in innate immunity by detecting bacterial peptidoglycan fragments and other danger signals.
• Specifically recognizes and binds gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP), a dipeptide present in Gram-negative bacterial peptidoglycan, and preferentially binds tripeptide-containing muropeptides (MurNAc-TriDAP/TriDAP).
  Related references:
  PMID:12791997 PMID:12796777 PMID:12871942 PMID:16211083
• Ligand binding triggers NOD1 oligomerization and recruitment of the proximal adapter RIPK2, which becomes polyubiquitinated and acts as a scaffold for downstream effectors, activating the NF-κB and MAP kinase signaling pathways.
  Related references:
  PMID:12791997 PMID:12796777 PMID:17054981 PMID:10880512 PMID:19043560
• Acts as a regulator of antiviral responses by modulating the formation of IFIH1-MAVS and TRAF3-MAVS complexes, enhancing the production of type I interferons.
  Related references:
  PMID:32169843
• Involved in autophagy regulation via interaction with ATG16L1, potentially recruiting it to bacterial entry sites (By similarity).
• Senses endoplasmic reticulum stress by binding the cytosolic metabolite sphingosine-1-phosphate, initiating an inflammatory response.
  Related references:
  PMID:27007849 PMID:33942347
• Highly expressed in adult heart, skeletal muscle, pancreas, spleen, and ovary; also detected in placenta, lung, liver, kidney, thymus, testis, small intestine, and colon.
  Related references:
  PMID:10224040
• Localizes to the cell membrane (including apical and basolateral regions) and the cytoplasm; contains leucine-rich repeats (LRRs) and a nucleotide-binding domain (NBD), and undergoes palmitoylation and ubiquitination.
• Exists in multiple isoforms: isoform 1 efficiently binds iE-DAP, whereas an alternative isoform lacks this activity, highlighting functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–270) of human NOD1; this region is shared among some isoforms, so the antibody may detect multiple splice variants. Validate isoform specificity in your experimental system.
• For Western blot, a major band at approximately 108 kDa is expected. Use NOD1-positive lysates (e.g., THP-1, HeLa) as positive controls, and optimize protein loading and transfer conditions.
• For immunohistochemistry (IHC-P), employ standard antigen retrieval methods (e.g., citrate buffer, pH 6.0) and titrate the antibody to achieve optimal signal-to-noise ratio.
• Cross-reactivity with mouse and rat NOD1 is confirmed; this enables testing in common rodent models. Perform in-house verification for your specific tissue or cell type.
• In ELISA, use a direct or capture format and include appropriate positive and negative controls; the antibody may be used as a detection reagent when paired with a suitable capture partner.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is committed to accelerating your innate immunity and infectious disease research by providing one-stop access to a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets like NOD1. With end-to-end support from concept to clinic, our technical consulting services help optimize your diagnostic development pipeline. Explore NOD1 products and related innate immune receptor tools to advance your projects efficiently.