Anti-NOC4L Rabbit Polyclonal Antibody for WB and ELISA - Q9BVI4

Core Product Specifications and Parameters

Biological Background: NOC4L Function and Localization

• NOC4L (Nucleolar complex protein 4 homolog) is also known as NOC4-like protein, NET49, UTP19, or nucleolar complex-associated protein 4-like protein.
• It is encoded by the NOC4L gene in humans (Gene ID: 79050) and serves as a homolog of the yeast nucleolar complex protein Noc4p.
• The protein is localized to the nucleolus and the nucleus membrane, and possesses a transmembrane helix, as indicated by keyword annotations.
• NOC4L has a molecular weight of approximately 58 kDa (UniProt reported mass: 58,468 Da) and has been identified in proteomics studies.
• The UniProt entry includes keywords: Membrane, Nucleus, Transmembrane, Transmembrane helix, Reference proteome, and 3D-structure, reflecting its structural features and experimental characterization.
• No tissue specificity or functional annotations are provided in the reference UniProt record; the protein's role may be inferred from its yeast homolog's involvement in ribosome biogenesis, though direct functional studies in human are lacking.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 260 amino acids of human NOC4L, which may facilitate detection of both full-length and potential processed forms.
• For Western blot, use whole cell lysates from human cell lines (e.g., HeLa) and expect a band near 58 kDa; include appropriate positive and negative controls.
• In ELISA, optimize coating antigen concentration and antibody dilution for linearity; consider using recombinant NOC4L protein as a standard.
• As a polyclonal antibody, lot-to-lot consistency should be verified; pretreatment with blocking buffers can reduce non-specific binding.
• Ensure sample preparation preserves membrane and nucleolar localization if targeting native conformations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-NOC4L polyclonal antibody supports research into nucleolar dynamics and membrane-associated nuclear proteins. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.