Anti-NOB1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9ULX3

Core Product Specifications and Parameters

Biological Background: NOB1 Function and Localization

• NOB1 (RNA-binding protein NOB1), also known as phosphorylation regulatory protein HP-10 or protein ART-4, is a 47 kDa protein encoded by the NOB1 gene in humans (GeneID: 28987, UniProt: Q9ULX3) and is conserved across several species.
• It functions as an endonuclease essential for the processing of 20S pre-rRNA precursor into mature 18S rRNA, a critical step in the biogenesis of the 40S ribosomal subunit (By similarity).
• Additionally, NOB1 may play a role in general mRNA degradation pathways (Probable).
• Subcellularly, NOB1 localizes predominantly to the nucleus (UniProt Q9ULX3).
• The protein contains a zinc-finger domain and binds zinc, classifying it as a metal-binding phosphoprotein (based on keywords).
• Tissue expression profiling indicates that NOB1 is detected in liver, lung, placenta, endothelial cells, and spleen (UniProt).

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal 1-230 region of human NOB1 (NP_054781.1). When using this antibody, consider that epitope recognition may be influenced by post-translational modifications or protein-protein interactions that mask this region.
• For Western blot, a band around 47 kDa is expected; however, validate the blotting conditions in your specific sample matrix to minimize non-specific binding.
• In immunofluorescence/ICC, optimize permeabilization and fixation steps to allow nuclear access, as NOB1 predominantly localizes to the nucleus.
• For ELISA, determine the optimal coating concentration and antibody dilution through checkerboard titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing solutions for IVD manufacturers and researchers, offering validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents for ribosome biogenesis and RNA processing studies. Leverage our supply chain to secure high-quality NOB1 antibodies and complementary raw materials for your diagnostic assay development.