Anti-nNOS Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - P29475

Core Product Specifications and Parameters

Biological Background: nNOS Function and Localization

• nNOS (Nitric Oxide Synthase 1) is a constitutive enzyme primarily responsible for the production of nitric oxide (NO) in neurons, where NO acts as a neurotransmitter and retrograde messenger in the nervous system.
• Beyond neuronal signaling, nNOS exhibits peptidyl-cysteine S-nitrosylase activity, mediating cysteine S-nitrosylation of cytoplasmic target proteins like SRR, thereby contributing to post-translational regulation.
• Encoded by the NOS1 gene, multiple isoforms arise through alternative splicing; Isoform 1 is ubiquitously expressed (brain, skeletal muscle, testis, lung, kidney), while Isoform 3 is testis-specific and Isoform 4 is detected in testis, skeletal muscle, lung, and kidney.
• Subcellularly, nNOS localizes to the cell membrane, including the sarcolemma in muscle cells, and to dendritic spines in neurons, positioning it for precise signaling.
• The protein is a heme-containing flavoprotein that binds calmodulin, FAD, FMN, NADPH, and iron, requiring multiple cofactors for its oxidoreductase activity.
• Post-translational modifications include phosphorylation and ubiquitination, which may regulate its activity, stability, or localization.
• As a key enzyme in nitric oxide signaling, nNOS is implicated in neurotransmission, synaptic plasticity, and muscle contractility.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 1335–1434), a region conserved across human, mouse, and rat, consistent with the reported cross-reactivity; consider sequence alignment if using in other species.
• For Western blot (WB), the high molecular weight of nNOS (~161 kDa) may necessitate extended transfer times and optimized gel percentage; validate detection in relevant tissue or cell lysates.
• In immunofluorescence (IF) applications, anticipate staining at the cell membrane, sarcolemma, or dendritic spines; co-staining with compartment-specific markers can aid localization.
• For ELISA, standard peptide or protein-based protocols should be applicable; verify specificity using recombinant nNOS or a competitive peptide blocking assay if non-specific signals are a concern.
• As a rabbit monoclonal antibody, it offers high lot-to-lot consistency; nevertheless, each new lot should be titrated in the intended assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs by providing a complete range of high-quality IVD raw materials for neurotransmitter and signaling targets like nNOS. In addition to this validated rabbit monoclonal antibody, we offer customized bulk antibody production, optimized antibody pairs, and ancillary reagents to streamline your assay development. Whether you need rare-target sourcing or technical consultation, our integrated supply chain accelerates your path from concept to clinic.