Anti-NNMT Rabbit PolymAb® for WB - P40261

Core Product Specifications and Parameters

Biological Background: NNMT Function and Localization

• NNMT (nicotinamide N-methyltransferase) catalyzes the N-methylation of nicotinamide using S-adenosyl-L-methionine, producing N1-methylnicotinamide. This reaction is a predominant pathway for nicotinamide/vitamin B3 clearance. Related references: PMID:21823666, PMID:23455543, PMID:8182091
• By consuming S-adenosyl-L-methionine, NNMT regulates cellular methylation potential, leading to global hypomethylation of repressive histone marks such as H3K27me3. This activity mediates widespread epigenetic and transcriptional changes. Related references: PMID:23455543, PMID:26571212, PMID:31043742
• In a developmental context, NNMT contributes to the low levels of repressive histone modifications that characterize pluripotent embryonic stem cells during the pre-implantation state. Related references: PMID:26571212
• NNMT also acts as a metabolic regulator in white adipose tissue (energy expenditure and polyamine flux) and liver (gluconeogenesis and cholesterol biosynthesis). Through its product N1-methylnicotinamide, it can stabilize SIRT1 deacetylase and regulate protein acetylation.
• The enzyme can N-methylate other pyridines structurally related to nicotinamide, suggesting a role in xenobiotic detoxification. Related references: PMID:30044909
• NNMT is predominantly expressed in the liver, with lower levels in kidney, lung, skeletal muscle, placenta, and heart. It is not detected in brain or pancreas.
• The protein localizes to the cytoplasm and belongs to the methyltransferase family, utilizing S-adenosyl-L-methionine as a methyl donor.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-264 of full-length human NNMT, providing a broad epitope recognition region. Consider this when designing truncation or domain mapping experiments.
• For Western blot (WB), a band at approximately 30kDa is expected. Validate in the relevant sample system, and use appropriate denaturing and reducing conditions for optimal detection.
• For immunofluorescence/immunocytochemistry (IF/ICC), we recommend optimizing fixation and permeabilization protocols based on the target cell type to preserve cytoplasmic localization.
• In immunoprecipitation (IP) assays, the rabbit monoclonal format ensures high specificity and compatibility with standard IP protocols.
• For ELISA, this antibody can be utilized in both direct and capture formats. Consider titrating the antibody concentration to establish the optimal signal-to-noise ratio.
• Cross-reactivity is confirmed for human, mouse, and rat NNMT. For other species, verify sequence homology beforehand.

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