Anti-NNMT Rabbit Polyclonal Antibody for WB, IHC-P, IF-P, ELISA - P40261

Core Product Specifications and Parameters

Biological Background: NNMT Function and Localization

• NNMT is a cytoplasmic methyltransferase that catalyzes the N-methylation of nicotinamide, using S-adenosyl-L-methionine (SAM) as a methyl donor, to produce N1-methylnicotinamide (MNA) and S-adenosyl-L-homocysteine (SAH). This constitutes a major clearance pathway for nicotinamide/vitamin B3. Related references: PMID:21823666 PMID:23455543 PMID:8182091
• By consuming SAM, NNMT reduces the cellular methylation potential, directly limiting SAM availability for other methyltransferases. This activity actively mediates genome-wide epigenetic reprogramming, notably through hypomethylation of repressive histone marks such as H3K27me3. Related references: PMID:23455543 PMID:26571212 PMID:31043742
• In early development, NNMT contributes to the low levels of repressive histone marks that characterize the pluripotent pre-implantation state of embryonic stem cells. Related references: PMID:26571212
• NNMT functions as a metabolic regulator in white adipose tissue, influencing energy expenditure, hepatic gluconeogenesis, and cholesterol biosynthesis. In white adipocytes, it regulates polyamine flux and, by consuming nicotinamide, controls NAD+ levels through the salvage pathway.
• The product N1-methylnicotinamide further regulates protein acetylation in hepatocytes by repressing ubiquitination and increasing the stability of SIRT1 deacetylase.
• NNMT can also N-methylate other pyridine compounds structurally related to nicotinamide, implicating it in xenobiotic detoxification. Related references: PMID:30044909
• Subcellular localization: NNMT is predominantly cytoplasmic.
• Tissue expression: NNMT is predominantly expressed in the liver, with lower levels in kidney, lung, skeletal muscle, placenta, and heart. It is not detected in brain or pancreas.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against full-length recombinant human NNMT (amino acids 1-264). Based on cross-reactivity data, it is expected to detect the endogenous protein in human, mouse, and rat samples.
• For western blotting (WB), the predicted molecular weight of NNMT is approximately 30 kDa. Consider using appropriate gel percentage and loading controls when analyzing tissue lysates, particularly from liver where NNMT is highly expressed.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), validate the staining pattern in tissues known to express NNMT, such as liver sections, and include proper negative controls to confirm specificity.
• In ELISA applications, optimize the coating antigen and antibody concentration to achieve the desired sensitivity.
• Given NNMT’s role in methylation and epigenetics, this antibody may be useful in studying changes in NNMT expression under various metabolic or developmental conditions.

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