Anti-NNMT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P40261

Core Product Specifications and Parameters

Biological Background: NNMT Function and Localization

• NNMT is a cytoplasmic methyltransferase that catalyzes the N-methylation of nicotinamide, a key step in the clearance of vitamin B3 (nicotinamide), using S-adenosyl-L-methionine (SAM) as a methyl donor.
  Related references: PMID:21823666 PMID:23455543 PMID:8182091
• By consuming SAM, NNMT regulates cellular methylation potential and mediates epigenetic modifications, notably reducing repressive histone mark H3K27me3, thereby promoting global transcriptional changes.
  Related references: PMID:23455543 PMID:26571212 PMID:31043742
• NNMT contributes to the pluripotent state of embryonic stem cells by limiting H3K27me3 levels, characteristic of the pre-implantation epigenetic landscape.
  Related references: PMID:26571212
• As a metabolic regulator, NNMT influences white adipose tissue energy expenditure, hepatic gluconeogenesis, and cholesterol biosynthesis, partly by affecting polyamine flux and NAD+ salvage pathways.
• NNMT can N-methylate other pyridine compounds structurally related to nicotinamide, suggesting a role in xenobiotic detoxification.
  Related references: PMID:30044909
• Predominantly expressed in the liver, with lower levels in kidney, lung, skeletal muscle, placenta, and heart; not detected in the brain or pancreas.
• Post-translational modifications include acetylation and citrullination, and the protein is a well-characterized reference proteome member with direct sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein spanning the full-length human NNMT (aa 1–264). This design is expected to generate antibodies recognizing the native protein structure; however, empirical validation is recommended for each application.
• For Western blotting, a band around 30 kDa is expected. Optimize antibody dilution and detection conditions using appropriate positive controls (e.g., liver lysate) and consider subcellular fractionation to enrich cytoplasmic proteins.
• In immunofluorescence/ICC, NNMT is cytoplasmic. Use appropriate fixation and permeabilization protocols to preserve cytoplasmic antigens, and validate with known negative tissues (e.g., brain).
• For ELISA applications, direct or sandwich formats can be tested, with careful titration of the coating antigen and antibody. Cross-reactivity with mouse and rat NNMT may allow multi-species assay development, but sequence homology should be verified.
• No pre-validated dilutions or performance data are provided; every experimental system requires individual optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by providing one-stop access to validated antibodies, antigen sources, and ancillary reagents for diagnostic development. This anti-NNMT polyclonal antibody is part of our extensive portfolio supporting epigenetic and metabolic research applications. Our supply capabilities extend to monoclonal/polyclonal antibody pairs, bulk reagent sourcing, and rare target raw-material identification, all backed by technical consultation to help move your projects from concept to clinic.