Anti-NNMT Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P40261

Core Product Specifications and Parameters

Biological Background: NNMT Function and Localization

• NNMT catalyzes the N-methylation of nicotinamide, producing N1-methylnicotinamide, a major pathway for nicotinamide clearance. Related references: PMID:21823666 PMID:23455543 PMID:8182091

• By consuming the methyl donor S-adenosyl-L-methionine, NNMT regulates the cellular methylation potential and influences epigenetic modifications, including H3K27me3 hypomethylation. Related references: PMID:23455543 PMID:26571212 PMID:31043742

• NNMT contributes to the low repressive histone marks in pluripotent embryonic stem cells, supporting the pre-implantation state. Related references: PMID:26571212

• NNMT acts as a metabolic regulator in white adipose tissue and liver, influencing energy expenditure, gluconeogenesis, cholesterol biosynthesis, and protein acetylation via SIRT1 stabilization.

• NNMT also methylates other pyridine substrates, suggesting a role in xenobiotic detoxification. Related references: PMID:30044909

• Subcellularly, NNMT is localized in the cytoplasm.

• NNMT is predominantly expressed in the liver, with lower levels in kidney, lung, skeletal muscle, placenta, and heart; undetectable in brain and pancreas.

• NNMT undergoes post-translational modifications including acetylation and citrullination.

Experimental Guidance and Technical Tips

• For Western blot, a band around 30 kDa is expected; consider using a suitable gel concentration for low molecular weight proteins. Validate in your sample system and optimize antibody dilution.
• For immunofluorescence, standard fixation/permeabilization protocols may be applicable; we recommend testing different conditions to achieve optimal nuclear and cytoplasmic staining.
• For ELISA, both direct and indirect formats can be explored; pairing with a matched detection antibody may enhance sensitivity.
• Due to predicted cross-reactivity with mouse and rat based on sequence similarity, always verify specificity in the relevant species using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supplier offering comprehensive solutions for diagnostic research and development. This anti-NNMT polyclonal antibody is part of our expanding portfolio of validated antibodies supporting studies in nicotinamide metabolism, epigenetic regulation, and metabolic disorders. In addition to high-quality antibodies, we provide optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—all from a single partner.