Anti-NNMT Rabbit Monoclonal Antibody for WB, ELISA - P40261

Core Product Specifications and Parameters

Biological Background: NNMT Function and Localization

• NNMT catalyzes the N-methylation of nicotinamide using S-adenosyl-L-methionine, forming N1-methylnicotinamide and S-adenosyl-L-homocysteine. This reaction represents a predominant nicotinamide/vitamin B3 clearance pathway. Related references: PMID:21823666 PMID:23455543 PMID:8182091
• By consuming S-adenosyl-L-methionine, NNMT regulates cellular methylation potential and mediates genome-wide epigenetic changes, including hypomethylation of repressive histone marks such as H3K27me3. Related references: PMID:23455543 PMID:26571212 PMID:31043742
• NNMT contributes to maintaining low levels of repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state. Related references: PMID:26571212
• In metabolic tissues, NNMT acts as a regulator of white adipose tissue energy expenditure, hepatic gluconeogenesis, and cholesterol biosynthesis. It also influences polyamine flux and NAD+ levels in adipocytes, and its product N1-methylnicotinamide stabilizes SIRT1 deacetylase in hepatocytes.
• Predominantly localized to the cytoplasm.
• Predominantly expressed in the liver, with lower expression in kidney, lung, skeletal muscle, placenta, and heart. Not detected in brain or pancreas.
• NNMT is subject to post-translational modifications including acetylation and citrullination, and belongs to the S-adenosyl-L-methionine-dependent methyltransferase family.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering full-length human NNMT (amino acids 1–264). This design may support recognition of both native and denatured NNMT in assays.
• Validated applications are western blot (WB) and ELISA. For WB, consider using reducing conditions and a 29 kDa expected band. For ELISA, optimize antibody pairing with appropriate detection reagents.
• Given NNMT's high expression in liver, hepatocyte-derived cell lines or liver tissue lysates may serve as suitable positive controls for antibody testing.
• This antibody is specific to human NNMT; cross-reactivity with other species has not been evaluated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for metabolic and epigenetic research. Explore our NNMT antibody for your next assay development project.