Anti-NMU Polyclonal Antibody for WB, ELISA - P48645

Core Product Specifications and Parameters

Biological Background: NMU (Neuromedin-U) Function and Localization

• NMU (Neuromedin-U) is a multifunctional neuropeptide that acts via two G protein-coupled receptors, NMUR1 and NMUR2, to regulate feeding, energy balance, blood pressure, smooth muscle contraction, hormone release, and inflammatory responses. Related references: PMID:15331768 PMID:35440625 PMID:15585845 PMID:33857254

• Upon binding to NMUR1 or NMUR2, the receptors couple to Galpha proteins GNAQ/Gaq or GNA11/Ga11 and GNAI1/Gai subunits, leading to phospholipase C activation and calcium mobilization (via Gq/11) or inhibition of cAMP accumulation (via Gi). Related references: PMID:15331768

• In pancreatic beta cells, NMU regulates insulin secretion through NMUR1 coupled to GNAI2 and GNAO1, resulting in reduced intracellular Ca2+ influx and cAMP levels. Related references: PMID:33857254

• Through NMUR1 in Th2-type T cells, NMU enhances type 2 cytokine secretion and modulates inflammatory responses via activation of PLC-calcineurin-MEK and PI3K signaling pathways. Related references: PMID:15585845

• The precursor protein is processed by signal peptide cleavage and propeptide removal, yielding biologically active neuromedin-U peptides; it is subject to post-translational modifications including amidation and oxidation.

• NMU is expressed throughout the enteric nervous system with highest levels in the jejunum, and also in the gastrointestinal tract. Related references: PMID:7619205

• Subcellular localization is secreted.

Experimental Guidance and Technical Tips

• The immunogen sequence is conserved across human, mouse, and rat, consistent with reported cross-reactivity; validate in your specific sample system.
• For Western blot, consider optimizing blocking and antibody dilution; a 20 kDa band is expected but may shift due to post-translational modifications.
• ELISA may be used for detection of NMU peptides in biological fluids; ensure sample preparation prevents degradation.
• Pre-adsorption with excess peptide immunogen can be used as a specificity control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive sourcing solution for IVD raw materials. For neuropeptide signaling and metabolic disease research, we offer validated antibodies, matched pairs, and ancillary reagents for biomarker assay development. Our team can also assist in sourcing rare or custom raw materials to accelerate your assay pipeline.