Anti-NMNAT3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96T66

Core Product Specifications and Parameters

Biological Background: NMNAT3 Function and Localization

• Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3 (NMNAT3), also known as PNAT-3, is a member of the NMNAT family involved in pyridine nucleotide biosynthesis.
• Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP; can also use nicotinic acid mononucleotide (NaMN) with equal efficiency and GTP/ITP as nucleotide donors. Catalyzes the reverse pyrophosphorolytic cleavage of NAD(+). Related references: PMID:16118205, PMID:17402747, PMID:26616331
• Protects against axonal degeneration following injury and may be involved in maintaining axonal integrity. Related references: PMID:16118205, PMID:17402747
• Functions as a stress-response chaperone that prevents toxic protein aggregation, independent of its NAD(+) synthesis activity. Related references: PMID:18344983
• Localizes predominantly to the mitochondrion.
• Expressed in lung and spleen with lower levels in placenta and kidney.
• Alternative splicing produces multiple isoforms; keywords include ATP-binding, magnesium, transferase, and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–215 of human NMNAT3, covering the N-terminal region; this polyclonal antibody may recognize multiple isoforms or splice variants.
• For Western blot (WB), the predicted protein weight is approximately 28kDa; validate band specificity using appropriate positive controls (e.g., mitochondrial fractions from lung or spleen lysates).
• In immunofluorescence (IF/ICC), expect mitochondrial staining; consider co-staining with a mitochondrial marker to confirm localization.
• ELISA performance may vary; optimize coating antigen and antibody dilution using the recombinant immunogen or native protein.
• Cross-reactivity with mouse and rat enables use in common model organisms; verify species-specific reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-NMNAT3 antibody supports research in NAD+ metabolism, neurodegeneration, and mitochondrial biology. Leverage CamelBio’s supply network for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic.