Anti-NMNAT2 Rabbit Polyclonal Antibody for WB and ELISA - Q9BZQ4

Core Product Specifications and Parameters

Biological Background: NMNAT2 Function and Localization

• Encoded by the NMNAT2 gene, nicotinamide/nicotinate mononucleotide adenylyltransferase 2 is a critical enzyme in pyridine nucleotide biosynthesis, catalyzing the conversion of nicotinamide mononucleotide (NMN) and ATP into NAD+. Related references: PMID:16118205 PMID:17402747

• It also accepts the deamidated substrate nicotinic acid mononucleotide (NaMN) with lower efficiency and catalyzes the reverse pyrophosphorolytic cleavage of NAD+, NADH, and NaAD. Related references: PMID:16118205 PMID:17402747

• The enzyme cannot use triazofurin monophosphate as a substrate and fails to cleave phosphorylated dinucleotides such as NADP+, NADPH, and NaADP+. Related references: PMID:16118205 PMID:17402747

• NMNAT2 functions as an activator of mono-ADP-ribosylation by enhancing the catalytic activity of PARP16, leading to ribosome ADP-ribosylation. Related references: PMID:34314702

• As an axon maintenance factor, NMNAT2 delays Wallerian axon degeneration and is required for axon survival; its depletion triggers axon degeneration.

• Subcellularly, NMNAT2 localizes to the Golgi apparatus membrane, cytoplasmic vesicle membrane, cytoplasm, and is enriched in axonal projections.

• It is highly expressed in brain regions including cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe, and putamen, and is also present in heart, skeletal muscle, pancreas, and islets of Langerhans.

• Post‑translational modifications include palmitoylation, which supports membrane association, and ubiquitin conjugation; alternative splicing generates multiple protein isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–302 of human NMNAT2, covering nearly the full‑length protein; this broad epitope coverage may confer reactivity against multiple isoforms and orthologs (human, mouse, rat).
• For Western blotting, consider using lysates from brain or neuronal cell lines where endogenous NMNAT2 expression is high; a band near 34 kDa is expected.
• Due to the Golgi/cytoplasmic localization, mild detergent lysis buffers (e.g., RIPA) are recommended, and samples should be validated in the relevant model system.
• In ELISA applications, the polyclonal nature can facilitate capture–detection pair development; however, batch‑to‑batch consistency should be confirmed for quantitative assays.
• The antibody is suitable for detecting NMNAT2 in human, mouse, and rat samples, but cross‑reactivity with other species should be tested empirically.

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