Anti-NMNAT1 Polyclonal Antibody for WB and ELISA - Q9HAN9

Core Product Specifications and Parameters

Biological Background: NMNAT1 Function and Localization

• NMNAT1 (Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1) is also known as nicotinamide-nucleotide adenylyltransferase 1 and nicotinate-nucleotide adenylyltransferase 1. It is a key enzyme in NAD+ biosynthesis.
• Catalyzes the ATP-dependent conversion of nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN) to NAD+ and NaAD+, respectively. Also accepts triazofurin monophosphate as a substrate and can perform the reverse pyrophosphorolytic cleavage of NAD+. Related references: PMID:17402747
• Functions in nuclear ATP generation together with PARP1, PARG, and NUDT5, providing energy for extensive chromatin remodeling. Related references: PMID:27257257
• Acts as a cofactor directing PARP1 catalytic activity to glutamate and aspartate residues on histones, influencing ADP-ribosylation.
• Localizes predominantly to the nucleus.
• Widely expressed with highest levels in skeletal muscle, heart, and kidney; also found in liver, pancreas, placenta, and throughout the brain.
• Protects against axonal degeneration following mechanical or toxic insults, a function that may require enzymatic activity but does not correlate with cellular NAD+ levels.
• Contains a zinc-binding motif and undergoes phosphorylation, as indicated by proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-150) of human NMNAT1. This region is likely exposed and may recognize both full-length and certain truncated forms. Consider verifying specificity in your sample system.
• Recommended for Western blot (WB) and ELISA. For WB, a band around 32 kDa is expected in human-derived lysates. Optimize antibody dilution and detection conditions, as polyclonal antibodies may require titration.
• Cross-reactivity is reported for human NMNAT1. If working with other species, validate cross-reactivity experimentally, as it is not guaranteed.
• Due to the target’s nuclear localization, consider appropriate lysis and fractionation methods to enrich nuclear proteins if signal is weak.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, technical services, and consulting—from early concept to clinical application. For NMNAT1-related metabolism and chromatin research, we offer validated rabbit polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. Our one-stop sourcing model streamlines procurement of rare-target raw materials, ensuring consistent quality and supply for your assay development pipelines.