Anti-NME8 Rabbit Polyclonal Antibody for WB, ELISA - Q8N427

Core Product Specifications and Parameters

Biological Background: NME8 Function and Localization

• NME8 (NM23-H8, TXNDC3, Sptrx-2) is a testis-specific member of the NME/NM23 family with intrinsic kinase activity.
  Related references:
  PMID:16313181
• In vitro, it lacks detectable nucleoside diphosphate kinase (NDPK) activity and disulfide bond-reducing activity.
  Related references:
  PMID:11737268 PMID:16313181
• It possesses a 3'-5' DNA exonuclease activity, suggesting involvement in DNA nucleolytic processing.
  Related references:
  PMID:16313181
• May be required during the final stages of sperm tail maturation, particularly in disulfide bonding of fibrous sheath (FS) proteins.
• Subcellular localization is primarily cytoplasmic.
• Expression is restricted to the testis, specifically in primary spermatocytes and round spermatids.
• Associated keywords include ciliopathy, primary ciliary dyskinesia, developmental protein, and spermatogenesis.

Experimental Guidance and Technical Tips

• The immunogen spans a large region (amino acids 299-588) of human NME8, which may provide recognition of multiple epitopes, enhancing sensitivity in denaturing Western blot applications.
• For WB, consider standard SDS-PAGE under reducing conditions with a protein weight of ~67 kDa; optimize primary antibody dilution using testis tissue lysates or relevant cell lines expressing NME8.
• In ELISA, use a recombinant NME8 protein as a coating antigen to establish a standard curve; confirm cross-reactivity with mouse samples if applicable.
• Validate antibody specificity in your experimental system by including appropriate controls (e.g., knockdown or knockout samples), as this is a polyclonal antibody.

CamelBio: Your One-Stop Sourcing Bridge

For researchers advancing spermatogenesis, ciliopathy, or DNA repair studies, CamelBio provides reliable one-stop access to IVD raw materials. We supply validated polyclonal antibodies, recombinant proteins, and ancillary reagents tailored to your assay development needs. Whether you require bulk quantities for diagnostic kit production or assistance sourcing rare target reagents, our team helps bridge the gap from research to clinical application.