Anti-NME6/NM23-H6 Polyclonal Antibody for WB, IHC-P, ELISA - O75414

Core Product Specifications and Parameters

Biological Background: NME6/NM23-H6 Function and Localization

• NME6, also known as nm23-H6 or IPIA-alpha, is a mitochondrial nucleoside diphosphate kinase (NDPK) that catalyzes the transfer of a gamma-phosphoryl group from ATP to nucleoside diphosphates via a phosphohistidine intermediate. Related references: PMID:10618642, PMID:34789336, PMID:37770567
• As a monomer, NME6 lacks NDPK activity, requiring complex formation with RCC1L for catalytic function. Related references: PMID:34789336
• NME6 participates in the regulation of mitochondrial nucleotide levels, mitoribosome biogenesis, and mitochondrial RNA abundances, and is crucial for supplying pyrimidine ribonucleoside triphosphates (rNTPs) for mitochondrial transcription. Related references: PMID:10618642, PMID:34789336, PMID:37770567
• It is required for the maintenance of mitochondrial DNA (mtDNA) when cytosolic import of pyrimidine deoxyribonucleoside triphosphates (dNTPs) is limited. Related references: PMID:37439264
• Subcellularly, NME6 localizes to the mitochondrial matrix and inner membrane.
• The protein is highly expressed in heart, placenta, skeletal muscle, kidney, prostate, ovary, intestine, and spleen, with varying levels across cell lines. Related references: PMID:10453732, PMID:10618642
• UniProt keywords include alternative splicing, apoptosis, ATP-binding, kinase, magnesium, mitochondrial inner membrane, and nucleotide metabolism.

Experimental Guidance and Technical Tips

• For Western blotting (WB), a band at ~21 kDa corresponding to endogenous NME6 is expected; consider mitochondrial enrichment for enhanced detection.
• When performing immunohistochemistry (IHC-P), optimize antigen retrieval; expect mitochondrial staining patterns.
• For ELISA, this antibody can be used for target capture or detection; validate pairing with a matched antibody in your assay system.
• As a recombinant protein fragment (aa 9–194) was used as immunogen, the antibody likely recognizes linear epitopes; performance in native conditions should be validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This Anti-NME6 polyclonal antibody is designed for investigating mitochondrial nucleotide metabolism and mtDNA maintenance. We offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets to streamline your assay development.