Anti-NME2/NM23B Polyclonal Antibody for WB, IHC-P, ELISA - P22392

Core Product Specifications and Parameters

Biological Background: NME2/NM23B Function and Localization

• NME2 functions as a nucleoside diphosphate kinase B, catalyzing the γ‑phosphoryl transfer from ATP to nucleoside diphosphates to maintain nucleoside triphosphate homeostasis. Related references: PMID:11121025, PMID:16313181, PMID:1851158
• It also acts as a histidine protein kinase, transferring the phosphoryl group from a phosphohistidine intermediate to target proteins such as GNB1 and KCNN4, regulating heterotrimeric G protein activation and calcium‑activated potassium channel activity. Related references: PMID:17157250, PMID:20946858
• NME2 is involved in transcriptional regulation by directly binding DNA and remodeling chromatin. Related references: PMID:11121025, PMID:11694515, PMID:19033359
• The protein stabilizes G‑quadruplex (G4) structures within the MYC gene promoter, acting as a single‑stranded DNA binding protein to activate MYC transcription independently of its kinase activity. Related references: PMID:19033359, PMID:19435876, PMID:25679041
• NME2 negatively regulates Rho activity through interaction with AKAP13. Related references: PMID:15249197
• Subcellular localization includes cytoplasm, nucleus, lamellipodia, ruffles, and perinuclear region.
• It is ubiquitously expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen spans the full‑length human NME2 protein (aa 1–152), likely contributing to the reported cross‑reactivity with mouse and rat due to high sequence conservation.
• For western blotting, the predicted molecular weight is ~17 kDa; ensure to include appropriate positive and negative controls and validate using known expression systems.
• When performing IHC‑P, consider testing multiple antigen retrieval protocols and antibody titrations on control tissue sections to achieve optimal staining.
• In ELISA applications, establish a standard curve and determine the optimal antibody concentration for quantitative detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw‑material sourcing, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development and research. For NME2/NM23B‑related projects in cancer biology, signal transduction, and transcriptional regulation, we offer tailored solutions for rare targets and custom production to advance your assay from concept to clinic.