Anti-NME2/NM23B Monoclonal Antibody for WB, ELISA - P22392

Core Product Specifications and Parameters

Biological Background: NME2/NM23B Function and Localization

• NME2 (Nucleoside diphosphate kinase B), also known as NM23-H2, PUF, or NDKB, catalyzes the transfer of gamma-phosphate from ATP to nucleoside diphosphates via a phosphohistidine intermediate, playing a central role in nucleoside triphosphate homeostasis. Related references: PMID:11121025, PMID:16313181, PMID:1851158
• It acts as a histidine protein kinase, phosphorylating key signaling proteins like the intermediate-conductance calcium-activated potassium channel KCNN4 at His-358, thereby activating Ca(2+) influx and promoting B and T cell activation. Related references: PMID:17157250, PMID:20946858
• Beyond its enzymatic activity, NME2 functions as a transcriptional regulator through direct DNA binding and chromatin remodeling. It specifically binds and stabilizes G-quadruplex (G4) structures in the MYC gene promoter, facilitating recruitment of single-strand DNA binding proteins and activating MYC transcription. Related references: PMID:11121025, PMID:11694515, PMID:19033359, PMID:19435876, PMID:25679041
• Together with NME1, NME2 may regulate acetyl-CoA usage between histone acetylation and fatty acid synthesis by targeting acetyl-CoA release at HAT-associated chromatin regions.
• NME2 negatively regulates Rho activity by interacting with AKAP13/LBC, providing a link to cytoskeletal dynamics. Related references: PMID:15249197
• Subcellular localization encompasses cytoplasm, nucleus, lamellipodia, ruffles, and perinuclear region, consistent with its multitasking roles in metabolism, signaling, and transcription.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 50–152) lies within a conserved region shared across human, mouse, and rat, supporting multi-species applications. Cross-reactivity has been confirmed for mouse and rat samples.
• For Western blotting, the predicted molecular weight is 17 kDa (17298 Da); use reduced samples and a suitable positive control such as HeLa or Jurkat whole-cell lysate.
• In ELISA, this monoclonal antibody can serve as either capture or detection reagent; optimization of coating buffer, blocker, and incubation conditions is recommended for individual assay formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined supply of IVD-grade antibodies and raw materials. This anti-NME2/NM23B monoclonal antibody supports investigations into cancer biology, transcriptional regulation, and nucleotide metabolism. We provide validated antibody pairs, bulk reagents, and custom sourcing for rare targets, helping you accelerate development from concept to clinical application.