Anti-NME1/NM23A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P15531

Core Product Specifications and Parameters

Biological Background: NME1/NM23A Function and Localization

• Nucleoside diphosphate kinase A (NDPK A, NME1, NM23-H1) is a ~17 kDa enzyme that catalyzes the transfer of a gamma-phosphoryl group from ATP to nucleoside diphosphates, maintaining nucleoside triphosphate homeostasis. Related references: PMID:10952986 PMID:14960567 PMID:16313181
• Additionally phosphorylates geranyl and farnesyl pyrophosphates, linking NME1 to isoprenoid metabolism. Related references: PMID:10952986
• Functions as a non-specific serine/threonine and histidine protein kinase, transferring phosphoryl groups to target proteins. Related references: PMID:8529641 PMID:9038158
• May act as a Mg²⁺-dependent single-stranded DNA endonuclease within the SET complex, cooperating with TREX1 to mediate apoptotic DNA fragmentation and suppress DNA repair; has been shown to cleave double-stranded DNA at specific promoter elements (e.g., PDGFA gene silencer) and may exhibit 3′-5′ DNA exonuclease activity. Related references: PMID:12628186 PMID:16818237 PMID:11694515 PMID:14960567 PMID:16313181
• Involved in the regulation of tumor metastasis and cellular differentiation; required for cell motility. Related references: PMID:8270257 PMID:25582197
• Localizes to the cytoplasm, nucleus, and cell membrane; ubiquitously expressed in normal tissues and tumor cell lines. Related references: PMID:12601555 PMID:16442775 PMID:10512675

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (aa 77–177); therefore, the antibody may recognize both full-length NME1 and potential splice variants or processed forms.
• For Western blotting, a band near 17 kDa is expected; whole cell lysates from human, mouse, or rat tissues are suitable starting samples.
• In IHC-P, antigen retrieval conditions should be optimized, and staining validated in known positive control tissues.
• For ELISA, the antibody can serve as a capture or detection reagent; optimal working concentrations must be determined by the end user.
• Cross-reactivity with other NME family members has not been evaluated; validate specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials. For NME1/NM23A-related projects—spanning oncology, nucleotide metabolism, and metastasis research—we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing service also encompasses rare target raw materials, helping you move from concept to clinic efficiently.