Anti-NME1/NM23A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P15531

Core Product Specifications and Parameters

Biological Background: NME1/NM23A Function and Localization

• NME1 (also known as NM23A, NDKA, NDPK-A, NM23-H1) is a nucleoside diphosphate kinase A that belongs to the NDK family.
• Catalyzes the transfer of a gamma-phosphoryl group from ATP to nucleoside diphosphates via a ping-pong mechanism, contributing to nucleoside triphosphate homeostasis. Related references: PMID:10952986, PMID:14960567, PMID:16313181
• Also functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups to target proteins. Related references: PMID:8529641, PMID:9038158
• May act as a Mg²⁺-dependent single-stranded DNA endonuclease in the SET complex, cooperating with TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes. Related references: PMID:12628186, PMID:16818237
• Localizes to the cytoplasm, nucleus, and cell membrane.
• Involved in the regulation of tumor metastasis and cellular differentiation, and required for cell motility. Related references: PMID:8270257, PMID:25582197
• Ubiquitously expressed in normal tissues and also detected in various tumor cell lines. Related references: PMID:12601555, PMID:16442775

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to the N-terminal region (aa 10-110) of human NME1; this region is highly conserved across species, which likely contributes to the observed cross-reactivity with mouse and rat.
• For Western blotting, the antibody detects a band at approximately 17 kDa. Optimize sample preparation and loading controls for your specific cell or tissue lysates.
• In immunocytochemistry/immunofluorescence, appropriate fixation and permeabilization methods should be tested, as NME1 localizes to multiple subcellular compartments.
• For ELISA applications, consider direct or indirect detection formats and validate with known positive and negative control antigens.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing IVD raw materials, offering this anti-NME1/NM23A antibody alongside a broad range of validated antibody pairs, recombinant proteins, and ancillary reagents. With expertise in cancer biology and nucleotide metabolism, we support bulk orders and custom procurement to accelerate your diagnostic and research workflows.