Anti-NME1/NM23A Rabbit Monoclonal Antibody for WB and ELISA - P15531

Core Product Specifications and Parameters

Biological Background: NME1/NM23A Function and Localization

• Nucleoside diphosphate kinase A (NDK A/NM23-H1) is a member of the nucleoside diphosphate kinase family, encoded by the NME1 gene, also known as NDPKA, NM23, and GAAD. It is ubiquitously expressed in human tissues and conserved across species.
• Core nucleoside triphosphate homeostasis: Catalyzes the transfer of a gamma-phosphoryl group from ATP to nucleoside diphosphates via a phosphohistidine intermediate, maintaining cellular NTP pools. Also phosphorylates geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP), linking to isoprenoid metabolism. Related references: PMID:10952986, PMID:14960567, PMID:16313181
• Additional kinase activities: Functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups to target proteins. Related references: PMID:8529641, PMID:9038158
• Role in DNA fragmentation and apoptosis: As part of the SET complex, NME1 acts as a Mg²⁺-dependent single-stranded DNA endonuclease, cooperating with TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes. It nicks one DNA strand, enabling TREX1 to process the free 3' end, enhancing DNA damage. May also function as a 3'-5' DNA exonuclease. Related references: PMID:12628186, PMID:16818237, PMID:14960567, PMID:16313181
• Tumor metastasis and cell motility: Involved in the regulation of tumor metastasis and cellular differentiation, and is required for cell motility. May control, with NME2, acetyl-CoA usage between histone acetylation and fatty acid synthesis at transcriptionally active chromatin regions. Related references: PMID:8270257, PMID:25582197
• Subcellular localization: Found in the cytoplasm, nucleus, and cell membrane, consistent with its diverse functions in signaling, nucleic acid metabolism, and membrane-associated processes.
• Post-translational modifications and keywords: The protein undergoes acetylation, phosphorylation, glutathionylation, and can form disulfide bonds. Keywords highlight its roles in kinase activity, nucleotide metabolism, apoptosis, and tumor suppression.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human NME1 (P15531). The resulting rabbit monoclonal antibody targets the N-terminal region, which is highly conserved across human, mouse, and rat.
• For Western blotting, consider using whole-cell lysates from human, mouse, or rat cell lines; the expected band weight is approximately 17 kDa under reducing conditions.
• For ELISA, the antibody can be used for direct binding assays with recombinant NME1 protein or the immunogen peptide; appropriate optimization of coating and detection conditions is recommended.
• While cross-reactivity with mouse and rat has been confirmed by the supplier, it is advisable to validate performance in your specific sample matrix and experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and institutes a comprehensive one-stop sourcing solution for IVD raw materials. This anti-NME1/NM23A monoclonal antibody exemplifies our commitment to supplying validated antibody products for cancer and nucleoside metabolism research. Complement your workflow with our range of bulk ancillary reagents, customized antibody pairs, and expert technical consulting to streamline your development from concept to clinic.