Anti-NME1/NM23A Polyclonal Antibody for WB, IF/ICC, ELISA - P15531

Core Product Specifications and Parameters

Biological Background: NME1/NM23A Function and Localization

• NME1/NM23A, also known as nucleoside diphosphate kinase A (NDK A) or NM23-H1, is a ubiquitously expressed member of the nucleoside diphosphate kinase family and was originally identified as a metastasis inhibitor.
• Core nucleoside diphosphate kinase activity: catalyzes the transfer of the gamma‑phosphoryl group from ATP to nucleoside diphosphates (GDP, CDP, UDP, etc.), maintaining nucleoside triphosphate homeostasis; also phosphorylates geranyl and farnesyl pyrophosphates, linking to isoprenoid metabolism. Related references: PMID:10952986, PMID:14960567, PMID:16313181
• Additional non‑specific kinase functions have been demonstrated, including serine/threonine and histidine protein kinase activities, suggesting roles in signal transduction. Related references: PMID:8529641, PMID:9038158
• NME1 possesses DNA nucleolytic activities: it acts as a Mg²⁺‑dependent single‑stranded DNA endonuclease within the SET complex, cooperating with TREX1 to mediate apoptotic DNA fragmentation, and also functions as a 3′‑5′ DNA exonuclease. Related references: PMID:12628186, PMID:16818237, PMID:11694515, PMID:14960567, PMID:16313181
• Required for cell motility and involved in the regulation of tumor metastasis and cellular differentiation. Related references: PMID:8270257, PMID:25582197
• Subcellular localization: found in the cytoplasm, nucleus, and cell membrane.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide encompassing amino acids 10‑110 of human NME1, a region predicted to be accessible and conserved; cross‑reactivity with rat implies epitope conservation across species.
• For western blot (WB), a band of approximately 17 kDa is expected under reducing conditions; optimize antibody dilution (e.g., 1:500–1:2000) and exposure times based on sample abundance.
• IF/ICC may require permeabilization protocols to detect nuclear and membrane‑associated pools; co‑staining with compartment‑specific markers is recommended.
• ELISA assays can be developed using the antibody as a capture or detection reagent; pairing with a matched monoclonal antibody may increase specificity for quantitative applications.
• Because NME1 is widely expressed, use appropriate positive (e.g., HEK293, HeLa) and negative (knockdown/knockout) controls to validate signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw‑material procurement, supporting diagnostic developers and researchers from early R&D through clinical application. This NME1/NM23A polyclonal antibody exemplifies our portfolio for cancer, metastasis, and nucleotide metabolism research. We offer a comprehensive range of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare‑target raw materials—streamlining your path from concept to clinic.