Anti-NMDAR2B Rabbit Monoclonal Antibody for WB, ELISA - Q13224

Core Product Specifications and Parameters

Biological Background: NMDAR2B Function and Localization

• NMDAR2B, also known as glutamate receptor ionotropic NMDA 2B (GluN2B) or NR2B, is a subunit of heterotetrameric NMDA receptors, which are ligand-gated cation channels with high calcium permeability. Related references: PMID:24272827, PMID:24863970, PMID:26875626
• Channel activation requires simultaneous binding of glutamate to GluN2B and glycine/D-serine to GluN1, along with postsynaptic depolarization to relieve the voltage-dependent Mg2+ block. Related references: PMID:24272827, PMID:24863970, PMID:26875626
• NMDAR2B plays a critical role in synaptic plasticity, particularly in hippocampus long-term depression (LTD), a cellular mechanism underlying learning and memory.
• This subunit contributes to unique channel kinetics, including sensitivity to pH, zinc, and allosteric modulators, distinguishing it from other GluN2 subunits. Related references: PMID:26875626, PMID:28095420, PMID:28126851
• Subcellularly, NMDAR2B localizes primarily to the postsynaptic cell membrane and dendrites; it is also found in late endosomes, lysosomes, and the cytoskeleton, indicating dynamic trafficking.
• Tissue expression is enriched in pyramidal neurons of the cerebral cortex and hippocampus, with lower levels in the basal ganglia.
• The protein undergoes post-translational modifications, including phosphorylation at Ser-1303 by DAPK1, which enhances channel activity and contributes to excitotoxicity during cerebral ischemia.
• Mutations in GRIN2B are associated with neurodevelopmental disorders such as epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal intracellular region (aa 1133–1484), suggesting that this antibody may recognize both full-length and C-terminal fragments of NMDAR2B.
• For Western blot, consider using mild lysis conditions to preserve receptor complexes; the predicted molecular weight is ~166 kDa, but N-glycosylation and phosphorylation may shift migration.
• Cross-reactivity with mouse and rat has been reported; validation in your species-specific sample system is recommended.
• This antibody is also suitable for ELISA; for capture or detection formats, appropriate buffer conditions should be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD manufacturers and research laboratories, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for neuroscience biomarker development. For rare targets like NMDAR2B, our team can assist in sourcing custom raw materials to accelerate your diagnostic pipeline from concept to clinic.