Anti-NMDAR2A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q12879

Core Product Specifications and Parameters

Biological Background: NMDAR2A Function and Localization

• NMDAR2A (GluN2A) is a subunit of N-methyl-D-aspartate (NMDA) receptors, which are heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg2+ (PubMed:20890276, PubMed:23933818, PubMed:23933819). Related references: PMID:20890276, PMID:23933818, PMID:23933819
• NMDA receptors play a critical role in synaptic plasticity, learning, and memory formation, contributing to the slow component of excitatory postsynaptic currents and long-term potentiation (By similarity).
• Channel activation requires binding of L-glutamate to the GluN2 subunit, glycine or D-serine to GluN1, and membrane depolarization to relieve Mg2+ block (PubMed:23933818, PubMed:23933819, PubMed:23933820). Related references: PMID:23933818, PMID:23933819, PMID:23933820
• Each GluN2 subunit confers distinct properties to NMDA receptors, including activation/deactivation kinetics, pH sensitivity, Ca2+ permeability, and sensitivity to allosteric modulators (PubMed:26875626, PubMed:26919761). Related references: PMID:26875626, PMID:26919761
• NMDAR2A localizes primarily to the postsynaptic membrane of excitatory synapses, particularly in dendritic spines, and is also found on cytoplasmic vesicle membranes.
• The protein undergoes post-translational modifications such as phosphorylation and glycosylation, and contains disulfide bonds and transmembrane helices; alternative splicing generates multiple isoforms.
• Mutations in the GRIN2A gene are associated with neurological disorders including epilepsy, highlighting its importance in normal brain function.
• According to UniProt keywords, NMDAR2A is a glycoprotein, phosphoprotein, and disease variant, and it binds ligands such as glutamate, glycine, and zinc, acting as a ligand-gated ion channel.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1130-1400) of human NMDAR2A. Verify epitope conservation in your target species (human, mouse, rat) via sequence alignment if cross-reactivity is critical.
• For Western blot, expect a band near 165 kDa. Optimize transfer conditions for high-molecular-weight proteins and include a prestained marker for orientation.
• In immunofluorescence/ICC, permeabilization is required to access intracellular epitopes, but consider that the receptor is primarily membrane-localized; include appropriate membrane markers or controls.
• Cross-reactivity with mouse and rat has been reported; always validate in your specific tissue or cell line with positive and negative controls.
• For ELISA, pair with a suitable capture/detection system and test serial dilutions of the antibody to establish an optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your diagnostic development. This anti-NMDAR2A antibody supports neuroscience research, and we can assist with custom sourcing for rare targets or large-volume needs, ensuring seamless integration from concept to clinic.