Anti-NLRX1 Rabbit Polyclonal Antibody (KO Validated) for WB, ELISA - Q86UT6

Core Product Specifications and Parameters

Biological Background: NLRX1 Function and Localization

• NLRX1, also known as NOD5, NOD9, and CLR11.3, is a member of the NLR family of innate immune receptors that localize to the mitochondria.
• Acts as a negative regulator of MAVS-mediated antiviral responses by inhibiting the virus-induced RLH–MAVS interaction. Related references: PMID:18200010
• Promotes autophagy through interaction with TUFM, recruiting ATG5 and ATG12 to the autophagy machinery. Related references: PMID:22749352
• Regulates MAVS-dependent NLRP3 inflammasome activation, leading to attenuation of apoptosis. Related references: PMID:27393910
• Enhances NF-kappa-B and JUN N-terminal kinase signaling by generating reactive oxygen species. Related references: PMID:18219313
• Exhibits sex-dependent regulation of viral-mediated inflammation and energy metabolism, contributing to sex differences observed in infectious and inflammatory diseases (By similarity).
• Localized to the mitochondrial outer membrane, where it interacts with key antiviral and metabolic pathways.
• Ubiquitously expressed, with strongest expression in mammary gland, heart, and muscle tissues; detected in HeLa, HEK293T, THP-1, HL-60, Raji, and Jurkat cell lines.

Experimental Guidance and Technical Tips

• The immunogen (aa 87-360) covers a segment of the N-terminal region including part of the NACHT domain, which may promote epitope accessibility in native conditions.
• For western blot, expect a specific band at approximately 108 kDa; additional bands from splice variants or post‑translational modifications may appear. Use HeLa or THP-1 lysates as positive controls.
• KO validation ensures high specificity; include NLRX1 knockout cell lysates as negative controls in your experiments.
• Cross-reactivity with mouse and rat enables applications in rodent models; validate expression patterns in relevant tissues.
• For ELISA, consider pairing with an antibody targeting a distinct epitope to develop sandwich immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by supplying validated antibodies, antigen pairs, and ancillary reagents for diagnostic developers. This NLRX1 polyclonal antibody supports research into antiviral immunity, autophagy, and inflammasome biology. Through our global network, we provide custom sourcing of rare targets, bulk reagent fulfillment, and integrated support from concept to clinic—all through a single partner.