Anti-Nlrp6 Rabbit Polyclonal Antibody for WB, ELISA - Q91WS2

Core Product Specifications and Parameters

Biological Background: NLRP6 Function and Localization

• NLRP6 (also known as Nalp6, Pypaf5, or Non-AVR) is a member of the NOD-like receptor (NLR) family that functions as the sensor component of the NLRP6 inflammasome, a supramolecular complex critical for innate immunity and inflammatory responses. Related references: PMID:21593405, PMID:30392956, PMID:32424362

• It acts as a pattern recognition receptor (PRR), recognizing pathogen-associated molecular patterns such as lipoteichoic acid (LTA) from Gram-positive bacteria and double-stranded RNA (dsRNA) from viruses, triggering inflammasome assembly through liquid-liquid phase separation (LLPS). Related references: PMID:26494172, PMID:30392956, PMID:34678144

• Upon activation, NLRP6 recruits the effector pro-inflammatory caspases CASP1 and CASP4, which catalyze the maturation and secretion of IL-1β and IL-18 into the extracellular milieu. Related references: PMID:30392956

• NLRP6 plays a central role in maintaining intestinal epithelial integrity and host defense, restricting infection by Gram-positive bacteria, enteric viruses, and the parasite C. tyzzeri; it also regulates goblet cell mucus secretion, antimicrobial peptide production, and gut microbiota composition. Related references: PMID:21565393, PMID:22763455, PMID:23696660, PMID:26494172, PMID:26638072, PMID:30392956, PMID:33372132, PMID:34678144

• It is highly expressed in the gastrointestinal tract, particularly in colonic myofibroblasts, epithelial cells, and goblet cells; also detected in hepatocytes, immune cells (T-cells, dendritic cells, macrophages), and tissues like lung, kidney, bladder, and gonads.

• Subcellularly, NLRP6 localizes to the cytoplasm, inflammasome complexes, and is associated with the cell membrane and nuclear membrane.

• Additional features include alternative promoter usage and splicing, an ATP-binding domain, a leucine-rich repeat (LRR) motif, and potential for ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 124-237 of mouse Nlrp6; consider assessing sequence homology between species if extended cross-reactivity is needed.
• This rabbit polyclonal antibody is recommended for Western blot (WB) and ELISA; optimize antibody dilution and detection conditions for your specific sample type and protocol.
• Due to the polyclonal nature, be aware of potential lot-to-lot variation; include appropriate positive and negative controls in your experiments.
• If studying inflammasome activation, consider using suitable cell treatments or infection models to induce NLRP6 expression and complex formation.

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CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and expert consulting—from concept to clinical application. Our catalog features validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets like Nlrp6. For your inflammasome and innate immunity research projects, count on CamelBio as your dependable procurement partner.