Anti-NLRP6 Rabbit Monoclonal Antibody for WB, ELISA - P59044

Core Product Specifications and Parameters

Biological Background: NLRP6 Function and Localization

• NLRP6 (also known as NALP6, PYPAF5, AVR, NAVR, PAN3, CLR11.4) is a member of the NOD-like receptor (NLR) family and functions as the sensor component of the NLRP6 inflammasome, which mediates inflammasome activation in response to pathogen-associated signals, leading to maturation and secretion of IL1B and IL18. Related references: PMID:30392956 PMID:34678144
• Acts as a pattern recognition receptor (PRR): it recognizes and binds specific microbial components such as lipoteichoic acid (LTA) from Gram-positive bacteria and double-stranded RNA (dsRNA) from viruses. It may also recognize lipopolysaccharide (LPS), though LPS is not a major activator. Related references: PMID:30392956 PMID:33377178 PMID:34678144 PMID:31932628
• Upon ligand binding, NLRP6 undergoes liquid-liquid phase separation (LLPS), which enhances multivalent interactions and is essential for inflammasome complex assembly. Related references: PMID:34678144
• The NLRP6 inflammasome recruits the effector pro-inflammatory caspases CASP1 and/or CASP4, which catalyze the maturation and secretion of IL1B and IL18, playing critical roles in innate immunity and inflammation. Related references: PMID:12387869 PMID:30392956 PMID:30674671
• In the intestine, NLRP6 is crucial for maintaining epithelial integrity and host defense against microbial infections, including restriction of Gram-positive bacteria via LTA recognition and enteric viruses through DHX15-mediated dsRNA sensing and GSDMD-dependent IL18 release. Related references: PMID:30392956 PMID:34161762 PMID:34678144
• Subcellularly, NLRP6 localizes to the cytoplasm, cytosol, inflammasome complex, cell membrane, and nuclear membrane.
• Tissue expression: NLRP6 is predominantly expressed in peripheral blood granulocytes and at lower levels in CD4+ and CD8+ T-cells, and is also detected in colonic myofibroblasts. Related references: PMID:12387869 PMID:21593405

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 793-892 of human NLRP6 (NP_612202.2). For Western blot, consider using a positive control like human peripheral blood leukocytes or colonic myofibroblasts, where endogenous NLRP6 is expressed. The predicted molecular weight is ~99 kDa; validate band specificity in your sample system.
• For ELISA, validate the antibody with recombinant NLRP6 or known positive samples. Pairing with appropriate detection antibodies may enhance assay performance; optimization of coating and blocking conditions is recommended.
• Cross-reactivity is reported for human only; verify reactivity with other species if required.
• Shipping on ice; store at -20°C upon receipt for long-term stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, supporting research from concept to clinic. As a trusted B2B partner, we streamline procurement for targets like NLRP6, enabling reliable supply for innate immunity and inflammasome assay development.