Anti-NLRP5 Polyclonal Antibody for WB, ELISA - P59047

Core Product Specifications and Parameters

Biological Background: NLRP5 Function and Localization

• NLRP5 (NACHT, LRR and PYD domains-containing protein 5), also known as MATER, NALP5, PAN11, PYPAF8, and CLR19.8, is a member of the NOD-like receptor (NLR) family and a key component of the subcortical maternal complex (SCMC) essential for early embryonic development.
• SCMC functions as a structural constituent of cytoplasmic lattices in oocytes and preimplantation embryos, storing maternal proteins critical for epigenetic reprogramming and preventing their degradation or premature activation.
• NLRP5 is required for the proper localization of cortical granules to the oocyte cortex via association with the cortical actin scaffold, and it facilitates cortical actin clearance prior to oocyte exocytosis to prevent polyspermy.
• Involved in regulating post-fertilization Ca2+ release and endoplasmic reticulum storage, as well as the localization of mitochondria to the cytoplasm and perinuclear region in oocytes and early embryos, independent of its role in cytoplasmic lattice formation.
• Subcellularly localizes to the cytoplasm, cytoplasmic vesicles (including cortical granules), mitochondria, nucleolus, and Golgi apparatus, reflecting its diverse roles in oocyte maturation and embryo development.
• Highly expressed in oocytes and early embryos, with lower expression in somatic tissues such as liver and spinal cord; also detected in cumulus cells. Related references: PMID:19542546 PMID:11925379 PMID:30877238
• Contains characteristic domains including a PYD (pyrin domain), NACHT domain with ATP-binding site, and leucine-rich repeats (LRRs), classifying it as a member of the NLRP subfamily; listed in databases with keywords such as ATP-binding, leucine-rich repeat, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 157-256 of human NLRP5 (NP_703148.4); sequence homology across species may support cross-reactivity with mouse and rat, but validation in the specific sample type is recommended.
• For Western blot, the predicted molecular weight of NLRP5 is approximately 134 kDa; ensure optimal transfer conditions for high-molecular-weight proteins (e.g., prolonged transfer times, low-percentage SDS-PAGE gels).
• In ELISA applications, coat with the target antigen and optimise antibody dilution to achieve a linear detection range suitable for your experimental system.
• Due to the protein's restricted expression in oocytes and early embryos, prepare lysates from appropriate biological samples and consider enrichment strategies if working with somatic tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions to provide one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities. For NLRP5—a key maternal factor in reproductive biology and developmental studies—we offer tailored raw-material solutions to support your assay development and research needs.