Anti-NLRP3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96P20

Core Product Specifications and Parameters

Biological Background: NLRP3 Function and Localization

• NLRP3 acts as the sensor component of the NLRP3 inflammasome, initiating complex assembly with PYCARD/ASC and CASP1, which leads to caspase-1-mediated cleavage and secretion of pro-inflammatory cytokines IL1B and IL18, as well as GSDMD-driven pyroptosis.
  Related references: PMID:16407889, PMID:18403674, PMID:18604214, PMID:27432880, PMID:23582325, PMID:28847925, PMID:31189953

• Inflammasome activation also triggers the extracellular release of HMGB1, amplifying inflammatory responses.
  Related references: PMID:22801494

• Under resting conditions, NLRP3 is maintained in an autoinhibited state via ADP binding.
  Related references: PMID:35114687

• A wide range of danger signals can activate NLRP3, including extracellular ATP, nigericin, reactive oxygen species, monosodium urate crystals, cholesterol crystals, amyloid-beta fibers, and environmental particulates.
  Related references: PMID:16407889, PMID:18403674, PMID:18604214

• Upon activation, NLRP3 is recruited to the microtubule organizing center (MTOC) and then to the dispersed trans-Golgi network (dTGN) membrane, where it binds phosphatidylinositol 4-phosphate and assembles the active inflammasome.
  Related references: PMID:36442502, PMID:39173637, PMID:30487600, PMID:34554188

• NLRP3 exhibits intrinsic ATPase activity.
  Related references: PMID:17483456

• In addition to its role in inflammasome formation, NLRP3 can function as a transcription factor, regulating Th2 cell differentiation and cytokine gene expression (By similarity).

• Subcellularly, NLRP3 localizes to the cytosol, inflammasome, Golgi apparatus membrane, mitochondria, endoplasmic reticulum, nucleus, and can also be secreted.

• Tissue expression is prominent in macrophages, dendritic cells, B- and T-cells, and it is also found in lung epithelial cells, chondrocytes, stratified squamous epithelia, and osteoblasts.
  Related references: PMID:33231615, PMID:34133077, PMID:11786556, PMID:17164409, PMID:23229815, PMID:12032915, PMID:17907925

Experimental Guidance and Technical Tips

• Immunogen covers the N-terminal region (aa 1-220) encompassing the PYRIN domain, suggesting reactivity with the full-length NLRP3 protein.
• For Western blotting, the calculated molecular weight is approximately 118 kDa; use low-percentage acrylamide gels and optimize transfer conditions for large proteins.
• In IF/ICC, NLRP3 may exhibit cytoplasmic and punctate perinuclear staining patterns; include appropriate controls and validate primary antibody dilution.
• This polyclonal antibody is applicable for ELISA; when developing sandwich assays, careful pairing with a detection antibody is recommended, and cross-reactivity should be assessed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, technical services, and consulting, supporting your journey from concept to clinic. This NLRP3 antibody supports research into inflammasome biology and innate immunity, and we can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare target materials. Partner with CamelBio for reliable, scalable reagent procurement tailored to diagnostic development.