Anti-NLRP3 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q96P20

Core Product Specifications and Parameters

Biological Background: NLRP3 Function and Localization

• NLRP3 (NACHT, LRR and PYD domains-containing protein 3), also known as cryopyrin, is a cytosolic pattern recognition receptor that serves as the sensor component of the NLRP3 inflammasome. Related references: PMID:16407889, PMID:18403674, PMID:18604214
• Upon activation by pathogens, crystals, or environmental particles, NLRP3 oligomerizes and recruits ASC and pro-caspase-1, forming an inflammasome complex that promotes caspase-1 activation; this leads to cleavage and secretion of pro-inflammatory cytokines IL-1β and IL-18, as well as gasdermin D-mediated pyroptosis. Related references: PMID:23582325, PMID:28847925, PMID:31189953
• The NLRP3 inflammasome also drives the unconventional secretion of the alarmin HMGB1, amplifying inflammatory responses. Related references: PMID:22801494
• Under resting conditions, NLRP3 is kept in an autoinhibited ADP-bound state; diverse stimuli that induce K+ efflux and membrane perturbation trigger its conformational activation. Related references: PMID:35114687
• Following activation, NLRP3 is transported to the microtubule organizing center (MTOC), where it is unlocked by NEK7, and then relocalizes to dispersed trans‑Golgi network (dTGN) vesicles by binding phosphatidylinositol 4‑phosphate (PtdIns4P), leading to active inflammasome assembly. Related references: PMID:36442502, PMID:39173637
• Independent of its inflammasome role, NLRP3 can act as a transcription regulator in T helper 2 (Th2) cells, binding DNA and promoting IL-4 expression to drive Th2 differentiation, with implications for allergic asthma and tumor growth.
• Subcellularly, NLRP3 localizes to the cytosol, mitochondria, endoplasmic reticulum, Golgi apparatus, and can be secreted; it is predominantly expressed in macrophages, dendritic cells, and epithelial barriers, with lower levels in monocytes and osteoblasts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–210 near the N-terminal PYD domain of human NLRP3; consider that the antibody may recognize an epitope in this region, which could be relevant when assessing truncated or mutant constructs.
• For Western blot, validate the antibody using human macrophage or peripheral blood mononuclear cell lysates, where NLRP3 expression is known; a band at ~118 kDa is expected.
• In immunoprecipitation experiments, ensure optimized lysis conditions to preserve NLRP3 complex integrity, as the target interacts with multiple proteins in the inflammasome.
• For ELISA applications, preliminary checkerboard titrations with recombinant NLRP3 or cell lysates are advised to establish optimal coating and detection concentrations.
• Since NLRP3 activation may involve post-translational modifications (e.g., ubiquitination, phosphorylation), consider treating cells with appropriate stimuli (e.g., LPS + nigericin) to observe changes in detection patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to high-quality IVD raw materials, serving as a procurement partner for diagnostic developers and research laboratories. For NLRP3 and inflammasome-related studies, we supply optimized monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents—backed by reliable sourcing capabilities to meet your assay development needs from concept to clinical application.