Anti-NLRP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NX02

Core Product Specifications and Parameters

Biological Background: NLRP2 Function and Localization

• NLRP2 is a member of the NOD-like receptor (NLR) family, characterized by an N-terminal pyrin domain (PYD), a central NACHT domain with ATPase activity, and C-terminal leucine-rich repeats (LRRs).
• It functions as a negative regulator of NF-kB signaling by suppressing TNF- and CD40-induced NFKB1 activity at the IKK complex level, inhibiting NFKBIA degradation.
• Upon association with the adaptor protein PYCARD (ASC), NLRP2 activates caspase-1 (CASP1), promoting the maturation and secretion of the pro-inflammatory cytokine IL-1β, likely as part of an inflammasome complex.
• The protein is expressed at high levels in lung, placenta, and thymus, and at lower levels in ovary, intestine, and brain. It is also highly abundant in oocytes and early embryos.
• Subcellularly, NLRP2 is localized predominantly in the cytoplasm.
• Keywords include alternative splicing, apoptosis, ATP-binding, disease variant, and leucine-rich repeat, indicating potential involvement in genetic disorders and cell death pathways.

Experimental Guidance and Technical Tips

• The immunogen spans residues 665–820 within the C-terminal LRR region, which may confer specificity among NLR family members; however, polyclonal antibodies can recognize multiple epitopes.
• For Western blot, the expected band is approximately 121 kDa; consider optimizing loading and transfer conditions for high-molecular-weight proteins.
• Validate the antibody in relevant sample systems, such as human and mouse tissues or cell lines expressing NLRP2.
• For immunofluorescence/ICC, confirm cytoplasmic localization and include appropriate controls to assess specificity.
• ELISA performance should be verified using the intended sample matrix, as polyclonal antibodies may exhibit lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials and technical support for diagnostics manufacturers and research laboratories. This anti-NLRP2 antibody supports studies in innate immunity, inflammasome biology, and NF-kB signaling. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare targets to accelerate your assay development from concept to clinic.