Anti-NLRP12 Rabbit Polyclonal Antibody for WB and ELISA - P59046

Core Product Specifications and Parameters

Biological Background: NLRP12 Function and Localization

• NLRP12 is also known as Monarch-1, PYPAF7, and RNO, encoded by the NLRP12 gene in humans.
• It functions as a potent negative regulator of inflammation, inhibiting both canonical and non-canonical NF-κB and ERK signaling pathways in dendritic cells and macrophages. Related references: PMID:15489334, PMID:17947705
• NLRP12 promotes bacterial tolerance by targeting NOD2 for proteasomal degradation. Related references: PMID:30559449
• In antiviral immunity, it suppresses RIG-I-mediated signaling by modulating ubiquitination of RIG-I, dampening type I interferon responses. Related references: PMID:30902577
• NLRP12 is predominantly expressed in peripheral blood leukocytes, especially eosinophils and granulocytes, with lower levels in monocytes.
• The protein localizes to the cytoplasm and contains leucine-rich repeats, a nucleotide-binding domain, and a PYRIN domain.
• Structurally, NLRP12 belongs to the NLR family, featuring an N-terminal PYRIN domain, a central NACHT domain, and C-terminal leucine-rich repeats.
• It has been implicated in mitigating obesity-related inflammation in adipose tissue (By similarity).

Experimental Guidance and Technical Tips

• For Western blotting, consider using appropriate positive controls such as human leukocyte lysates and verify the expected band size (~120 kDa).
• Optimize antibody dilution and blocking conditions for WB; inclusion of loading controls and molecular weight markers is essential.
• ELISA can be used for antibody titer determination or target detection; validate with recombinant NLRP12 protein or known positive samples.
• Ensure cross-reactivity validation in human-derived samples; note that reactivity in other species has not been tested.
• The immunogen corresponds to the C-terminal region (aa 862-1061); confirm epitope specificity if detecting isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research laboratories, offering validated antibodies, bulk ancillary reagents, and custom sourcing for specialized targets. For studies on inflammation and immune regulation, we provide reliable anti-NLRP12 polyclonal antibodies alongside complementary reagents to accelerate your assay development from concept to clinical application.