Anti-NLRP12 Polyclonal Antibody for WB, ELISA - P59046

Core Product Specifications and Parameters

Biological Background: NLRP12 Function and Localization

• NLRP12 is a potent mitigator of inflammation, primarily expressed in dendritic cells and macrophages, where it inhibits both canonical and non-canonical NF-κB and ERK activation pathways. Related references: PMID:15489334, PMID:17947705, PMID:30559449
• It functions as a negative regulator of NOD2 by targeting it for proteasomal degradation, thereby promoting bacterial tolerance. Related references: PMID:30559449
• NLRP12 also inhibits RIGI-mediated immune signaling against RNA viruses by reducing TRIM25-mediated 'Lys-63'-linked RIGI activation and enhancing RNF125-mediated 'Lys-48'-linked RIGI degradation. Related references: PMID:30902577
• It acts as a negative regulator of inflammatory response in adipose tissue, helping to mitigate obesity and obesity-associated diseases.
• Subcellularly, NLRP12 is localized in the cytoplasm.
• NLRP12 is predominantly expressed in peripheral blood leukocytes, especially eosinophils and granulocytes, with lower levels in monocytes.
• The protein contains key structural domains including a pyrin domain (PYD), a NACHT domain with ATP-binding, and C-terminal leucine-rich repeats (LRRs).
• Alternative splicing of NLRP12 produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 862–1061) targets the C-terminal region of human NLRP12, which encompasses the leucine-rich repeat (LRR) domain; confirm that the antibody recognizes full-length NLRP12 in your model system.
• For western blotting, expect a band at approximately 120 kDa; use a positive control such as peripheral blood leukocyte lysates or NLRP12-overexpressing cells, and validate with siRNA knockdown or knockout lysates when possible.
• This antibody is also applicable for ELISA; determine optimal coating concentrations and dilution factors using purified recombinant NLRP12 protein.
• Noted cross-reactivity with rat; verify species reactivity in rat-derived samples using appropriate positive and negative controls.
• As a polyclonal antibody, evaluate lot-to-lot consistency if used in long-term studies or diagnostic assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers investigating NLRP12 and inflammatory pathways, CamelBio provides reliable polyclonal antibodies like this one, along with customized sourcing options for rare targets. From early-stage research to clinical development, our one-stop supply chain supports diagnostic manufacturers and laboratories worldwide.