Anti-NLRP10 Rabbit Polyclonal Antibody - Q86W26

Core Product Specifications and Parameters

Biological Background: NLRP10 Function and Localization

• NLRP10 (also known as NOD8, PYNOD, NALP10) is a member of the NLR family containing an N-terminal PYD domain, a central NACHT domain, and C-terminal LRRs.
• Functions as a negative regulator of inflammasome signaling by inhibiting CASP1 autoprocessing, IL-1β secretion, and PYCARD-mediated apoptosis (PubMed:15096476). Related references: PMID:15096476
• Exhibits dual enzymatic activity: both ATPase and GTPase, suggesting involvement in nucleotide-dependent signaling (PubMed:23861819). Related references: PMID:23861819
• Contributes to innate immune responses against bacterial and fungal infections, including C. albicans and invasive bacteria (PubMed:22672233). Related references: PMID:22672233
• Subcellularly localized to the cytoplasm and cell membrane, indicating potential signaling roles at both sites.
• Highly expressed in epidermal layers and dermal fibroblasts; also found in heart, brain, skeletal muscle, and colon (PubMed:22672233, PubMed:15096476). Related references: PMID:22672233 PMID:15096476
• Plays a tissue-specific role in periodontal immunity by inducing IL1A via ERK activation in oral epithelial cells upon bacterial challenge (PubMed:28766990). Related references: PMID:28766990
• Involved in T-cell-mediated skin inflammation, underscoring its role in adaptive immunity at barrier surfaces.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein covering amino acids 1-300 of human NLRP10 (NP_789791.1), which encompasses the PYD domain; this design may aid discrimination from other NLR family members.
• For Western blotting, expect a band at approximately 75 kDa; verify using positive controls from human, mouse, or rat tissues known to express NLRP10.
• ELISA suitability is indicated; consider testing a range of antibody dilutions and appropriate blocking conditions for optimal signal-to-noise ratio.
• Cross-reactivity with human, mouse, and rat is reported; species-specific validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents. For NLRP10-related innate immunity studies, we offer customizable sourcing solutions for polyclonal and monoclonal antibodies, as well as bulk-order options to support scaled immunoassay development. Our end-to-end support spans target validation, technical consultation, and regulatory compliance, ensuring a seamless path from concept to clinic.