Anti-NLRC5 Rabbit Polyclonal Antibody for WB, ELISA - Q86WI3

Core Product Specifications and Parameters

Biological Background: NLRC5 Function and Localization

• NLRC5 (NOD4/NOD27/CLR16.1) is a member of the NLR (nucleotide-binding domain and leucine-rich repeat containing) family of proteins, functioning as an intracellular sensor in innate immunity.
• It acts as a probable regulator of the NF-kappa-B and type I interferon (IFN) signaling pathways, thereby modulating inflammatory and antiviral responses.
• May also regulate type II interferon signaling, contributing to homeostatic control of innate immunity.
• Localizes to the cytoplasm, where it likely interacts with signaling adaptors to modulate immune pathways.
• Contains characteristic domains including a nucleotide-binding domain (NBD) and leucine-rich repeats (LRRs), which are critical for its regulatory functions.
• Expressed in a variety of tissues including spleen, thymus, lung, brain, tonsil, heart, and prostate, suggesting broad roles in immune surveillance.
• Alternative splicing leads to multiple transcript variants, potentially expanding its functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 240 amino acids of human NLRC5; antibodies may recognize epitopes in this region, which could influence binding under different assay conditions.
• For Western blotting, a protein with a predicted molecular weight of ~205 kDa may require longer transfer times and careful protein extraction to ensure detection.
• ELISA experiments may benefit from optimization of antigen coating conditions and antibody titration to achieve optimal signal-to-noise ratios.
• Given the reported mouse cross-reactivity, this antibody may be suitable for studies in mouse models; however, further validation in target tissues is recommended.
• Since NLRC5 is involved in NF-kB and interferon signaling, consider stimulating immune cell lines (e.g., THP-1, RAW 264.7) to enhance expression prior to detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This NLRC5 polyclonal antibody supports research into innate immunity and inflammatory signaling pathways, offering a reliable tool for assay development. Beyond individual antibodies, CamelBio supplies validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and can source rare targets to streamline your IVD pipeline.