Anti-NLRC5 Polyclonal Antibody for WB, ELISA - Q86WI3

Core Product Specifications and Parameters

Biological Background: NLRC5 Function and Localization

• NLRC5 (Nucleotide-binding oligomerization domain-like receptor family caspase recruitment domain containing 5) is a member of the NLR family and is also known as CLR16.1, NOD27, and NOD4.
• It acts as a probable regulator of the NF-kappa-B and type I interferon signaling pathways, playing a key role in innate immunity and antiviral defense.
• NLRC5 may also modulate type II interferon signaling, contributing to homeostatic control of immune responses.
• The protein contains characteristic domains including leucine-rich repeats (LRRs) and a nucleotide-binding domain, with predicted ATP binding activity.
• NLRC5 is localized in the cytoplasm, as indicated by its subcellular distribution.
• It is expressed in various tissues including spleen, thymus, lung, brain, tonsil, heart, and prostate, suggesting tissue-specific immune functions.
• Alternative splicing generates multiple isoforms, adding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant human NLRC5 protein; the resulting polyclonal antibody may recognize multiple epitopes and can be used for both WB and ELISA.
• For Western blot, consider using whole cell lysates from human spleen or thymus, where NLRC5 expression is relatively high. For mouse cross-reactivity, validate with mouse spleen or thymus lysates.
• For ELISA, the antibody can be used as a capture or detection reagent; consider pairing with another anti-NLRC5 antibody or using recombinant NLRC5 protein as standard.
• Optimize antibody dilution and incubation conditions for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including validated antibodies such as this anti-NLRC5 polyclonal antibody. We offer one-stop access to antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for innate immunity and infectious disease research. Contact CamelBio for customized sourcing solutions and technical consultation.