Anti-NLRC4 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q9NPP4

Core Product Specifications and Parameters

Biological Background: NLRC4 Function and Localization

• NLRC4 (NLR family CARD domain-containing protein 4) is a member of the NLR protein family, characterized by an N-terminal CARD domain, a central NACHT domain, and C-terminal leucine-rich repeats (LRRs).
• It acts as a key inflammasome component that indirectly senses proteins from pathogenic bacteria and fungi, assembling an inflammasome complex to promote caspase-1 activation, cytokine production, and macrophage pyroptosis. Related references: PMID:15107016
• Upon activation, the NLRC4 inflammasome drives caspase-1-dependent maturation and release of pro-inflammatory cytokines IL-1β and IL-18.
• NLRC4 localizes predominantly in the cytoplasm and cytosol, and upon sensing bacterial ligands, it oligomerizes into the inflammasome complex.
• Alternative splicing generates multiple isoforms: isoform 1 is highly expressed in lung, leukocytes, lymph node, and colon; isoform 2 is ubiquitously expressed with enrichment in lung and spleen; isoform 4 is brain-specific.
• Post-translational modifications include phosphorylation, consistent with its regulation in innate immune signaling.
• Mutations in NLRC4 are linked to autoinflammatory syndromes such as familial cold autoinflammatory syndrome 4 (FCAS4) and autoinflammation with infantile enterocolitis (AIFEC).

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 85-170 of human NLRC4; verify sequence homology in mouse and rat to ensure expected cross-reactivity.
• For Western blotting, NLRC4 migrates at ~116 kDa; include positive controls (e.g., LPS-stimulated macrophage lysates) and optimize transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry, NLRC4 exhibits cytoplasmic staining, but inflammasome activation induces punctate aggregates; consider treating cells with bacterial components or flagellin to visualize this relocation.
• For ELISA, titrate the antibody and recombinant protein to establish optimal working concentrations, and use a matched antibody pair if available.
• This polyclonal antibody may detect multiple isoforms; confirm identity of bands by mass spectrometry or isoform-specific probes if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined one-stop channel for IVD raw materials, including validated antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-NLRC4 polyclonal antibody supports innate immunity and inflammasome research, with proven performance in WB, IF, and ELISA across human, mouse, and rat. Reach out to inquire about matched antibody pairs, conjugation services, or bulk supply needs to accelerate your assay development.