Anti-NLK Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9UBE8

Core Product Specifications and Parameters

Biological Background: NLK Function and Localization

• NLK (Nemo-like kinase) is a serine/threonine-protein kinase and member of the kinase family, also known as LAK1. It contains ATP-binding and metal-binding sites typical of protein kinases.
• In the non-canonical Wnt pathway, NLK acts as a positive effector downstream of WNT5A, TAK1, and HIPK2, influencing cell fate determination. Related references: PMID:15004007, PMID:15764709
• NLK negatively regulates canonical Wnt/β-catenin signaling by phosphorylating TCF7L2/TCF4 and LEF1, resulting in dissociation from DNA and LEF1 proteolysis, thereby suppressing Wnt target gene expression. Related references: PMID:12482967, PMID:21454679
• It functions as a negative regulator of Notch signaling through phosphorylation of NOTCH1, blocking assembly of the transcriptionally active ternary complex. Related references: PMID:20118921
• NLK phosphorylates and destabilizes MYB family transcription factors (MYB, MYBL1, MYBL2), inhibiting their transcriptional activity either by promoting proteolysis or disrupting coactivator binding. Related references: PMID:15082531
• In IL6/IL1B signaling, NLK phosphorylates STAT3 and cooperates with ATF5 to enhance C/EBP transcriptional activity, while also stabilizing ATF5 protein levels. Related references: PMID:15004007, PMID:15764709, PMID:25512613
• Under osmotic stress, NLK phosphorylates RPTOR, inhibiting mTORC1 lysosomal recruitment and downstream signaling. Related references: PMID:26588989
• NLK localizes to both the nucleus and cytoplasm, where it modulates transcription factor activity and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 400-500 of human NLK; this may provide high specificity in relevant applications.
• For Western blot, the expected molecular weight is approximately 58 kDa; consider appropriate positive and negative controls (e.g., NLK-expressing cell lines).
• For immunohistochemistry (paraffin), antigen retrieval and antibody titration optimization may improve staining; validate in tissues known to express NLK.
• ELISA performance depends on the assay format; consider direct or indirect setups with suitable blocking agents.
• As a rabbit monoclonal antibody, it is compatible with anti-rabbit IgG secondary antibodies; minimize cross-reactivity to other species.
• Avoid repeated freeze-thaw cycles; store according to the supplier’s recommendation.

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CamelBio provides diagnostic manufacturers and research laboratories with a seamless sourcing channel for high-quality IVD raw materials, including validated antibody pairs, bulk reagents, and specialized monoclonals like the anti-NLK antibody. Our integrated supply chain supports rare target procurement and technical consultation, covering every stage from concept to clinic. Contact us to streamline your next project’s raw-material needs in kinase signaling and cancer research.